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dc.creatorOstafe, Raluca
dc.creatorProdanović, Radivoje
dc.creatorCommandeur, Ulrich
dc.creatorFischer, Rainer
dc.date.accessioned2018-11-22T00:25:33Z
dc.date.available2018-11-22T00:25:33Z
dc.date.issued2013
dc.identifier.issn0003-2697
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1606
dc.description.abstractWe have developed a novel, ultra-high-throughput screening assay for the detection of cellulase activity based on fluorescence-activated cell sorting (FACS) and double emulsion technology. Cellulase activity is detected using a series of coupled enzymes, including hexose oxidase (HOx), which generates hydrogen peroxide from the reducing sugars released by cellulases in the presence of any natural or artificial substrate. The assay can be adapted to suit a microtiter plate format, but the highest throughput is achieved by using FACS to screen high-complexity cellulase clone libraries. Using this approach, we achieved a 12-fold enrichment of positive (cellulase-expressing) cells in cellulase reference libraries after just one sorting round.en
dc.publisherAcademic Press Inc Elsevier Science, San Diego
dc.relationExcellence Initiative by the German federal government
dc.rightsrestrictedAccess
dc.sourceAnalytical Biochemistry
dc.subjectCellulaseen
dc.subjectFluorescence assayen
dc.subjectHexose oxidaseen
dc.subjectVanadium bromoperoxidaseen
dc.subjectViPeren
dc.subjectFACSen
dc.subjectDouble emulsionsen
dc.titleFlow cytometry-based ultra-high-throughput screening assay for cellulase activityen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractФисцхер, Раинер; Остафе, Ралуца; Продановић, Радивоје; Цоммандеур, Улрицх;
dc.citation.volume435
dc.citation.issue1
dc.citation.spage93
dc.citation.epage98
dc.identifier.wos000315477600014
dc.identifier.doi10.1016/j.ab.2012.10.043
dc.citation.other435(1): 93-98
dc.citation.rankM22
dc.identifier.pmid23146590
dc.description.otherSupplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3494]
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84873985413
dc.identifier.rcubKon_2437


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