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dc.creatorPopović, Milica M.
dc.creatorProdanović, Radivoje
dc.creatorOstafe, Raluca
dc.creatorSchillberg, Stefan
dc.creatorFischer, Rainer
dc.creatorGavrović-Jankulović, Marija
dc.date.accessioned2018-11-22T00:30:48Z
dc.date.available2018-11-22T00:30:48Z
dc.date.issued2015
dc.identifier.issn0257-277X
dc.identifier.urihttps://cherry.chem.bg.ac.rs/handle/123456789/1668
dc.description.abstractHigh-throughput characterization of allergens relies often on phage display technique which is subject to the limitations of a prokaryotic expression system. Substituting the phage display platform with a yeast surface display could lead to fast immunological characterization of allergens with complex structures. Our objective was to evaluate the potential of yeast surface display for characterization of plant-derived food allergens. The coding sequence of mature actinidin (Act d 1) was cloned into pCTCON2 surface display vector. Flow cytometry was used to confirm localization of recombinant Act d 1 on the surface of yeast cells using rabbit polyclonal antisera IgG and IgE from sera of kiwifruit-allergic individuals. Immunological (dot blot, immunoblot ELISA and ELISA inhibition), biochemical (enzymatic activity in gel) and biological (basophil activation) characterization of Act d 1 after solubilization from the yeast cell confirmed that recombinant Act d 1 produced on the surface of yeast cell is similar to its natural counterpart isolated from green kiwifruit. Yeast surface display is a potent technique that enables fast immunochemical characterization of allergens in situ without the need for protein purification and offers an alternative that could lead to improvement of standard immunodiagnostic and immunotherapeutic approaches.en
dc.publisherHumana Press Inc, Totowa
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.relationFEBS Collaborative and Experimental Scholarship for Central and Eastern Europe
dc.rightsrestrictedAccess
dc.sourceImmunologic Research
dc.subjectActinidinen
dc.subjectAllergen characterizationen
dc.subjectCysteine proteaseen
dc.subjectKiwifruiten
dc.subjectYeast surface displayen
dc.titleYeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergensen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractГавровић-Јанкуловић, Марија; Сцхиллберг, Стефан; Поповић, Милица; Продановић, Радивоје; Фисцхер, Раинер; Остафе, Ралуца;
dc.citation.volume61
dc.citation.issue3
dc.citation.spage230
dc.citation.epage239
dc.identifier.wos000350295900006
dc.identifier.doi10.1007/s12026-014-8614-0
dc.citation.other61(3): 230-239
dc.citation.rankM22
dc.identifier.pmid25537533
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84925518006
dc.identifier.rcubKon_2814


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