High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source
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2015
Authors
Kekez, B. D.Gojgić-Cvijović, Gordana D.
Jakovljević, Dragica M.
Stefanović Kojić, Jovana R.
Marković, M. D.
Beškoski, Vladimir
Vrvić, Miroslav M.
Article (Published version)
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In this study, levan production by Bacillus licheniformis NS032 isolated from a petroleum sludge sample was investigated. High levan yield was obtained in a wide range of sucrose concentrations (up to 400 g/L) and, contrary to most levan-producing strains, using ammonium chloride as the sole N source. Interaction between sucrose, ammonium chloride, and initial pH of the medium in a low sucrose (60-200 g/L) and a high sucrose (300-400 g/L) system was analyzed by response surface methodology. According to the calculated model in the low sucrose system, maximum predicted levan yield was 47.8 g/L (sucrose 196.8 g/L, ammonium chloride 2.4 g/L, pH 7.0), while in the high sucrose system, levan yield was 99.2 g/L (sucrose 397.6 g/L, ammonium chloride 4.6 g/L, pH 7.4). In addition, protective effect of microbial levan against copper toxicity to Daphnia magna is observed for the first time. The acute toxicity (48 h EC50) of copper decreased from 0.14 to 0.44 mg/L by levan in concentration of 50 ...ppm.
Keywords:
Levan / Box-Behnken design / pH / Bacillus licheniformis / Response surface methodology / Copper / Daphnia magnaSource:
Applied Biochemistry and Biotechnology, 2015, 175, 6, 3068-3083Publisher:
- Humana Press Inc, Totowa
Funding / projects:
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3376
DOI: 10.1007/s12010-015-1475-8
ISSN: 0273-2289
PubMed: 25592434
WoS: 000350881100024
Scopus: 2-s2.0-84924053585
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Kekez, B. D. AU - Gojgić-Cvijović, Gordana D. AU - Jakovljević, Dragica M. AU - Stefanović Kojić, Jovana R. AU - Marković, M. D. AU - Beškoski, Vladimir AU - Vrvić, Miroslav M. PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1670 AB - In this study, levan production by Bacillus licheniformis NS032 isolated from a petroleum sludge sample was investigated. High levan yield was obtained in a wide range of sucrose concentrations (up to 400 g/L) and, contrary to most levan-producing strains, using ammonium chloride as the sole N source. Interaction between sucrose, ammonium chloride, and initial pH of the medium in a low sucrose (60-200 g/L) and a high sucrose (300-400 g/L) system was analyzed by response surface methodology. According to the calculated model in the low sucrose system, maximum predicted levan yield was 47.8 g/L (sucrose 196.8 g/L, ammonium chloride 2.4 g/L, pH 7.0), while in the high sucrose system, levan yield was 99.2 g/L (sucrose 397.6 g/L, ammonium chloride 4.6 g/L, pH 7.4). In addition, protective effect of microbial levan against copper toxicity to Daphnia magna is observed for the first time. The acute toxicity (48 h EC50) of copper decreased from 0.14 to 0.44 mg/L by levan in concentration of 50 ppm. PB - Humana Press Inc, Totowa T2 - Applied Biochemistry and Biotechnology T1 - High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source VL - 175 IS - 6 SP - 3068 EP - 3083 DO - 10.1007/s12010-015-1475-8 ER -
@article{ author = "Kekez, B. D. and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Stefanović Kojić, Jovana R. and Marković, M. D. and Beškoski, Vladimir and Vrvić, Miroslav M.", year = "2015", abstract = "In this study, levan production by Bacillus licheniformis NS032 isolated from a petroleum sludge sample was investigated. High levan yield was obtained in a wide range of sucrose concentrations (up to 400 g/L) and, contrary to most levan-producing strains, using ammonium chloride as the sole N source. Interaction between sucrose, ammonium chloride, and initial pH of the medium in a low sucrose (60-200 g/L) and a high sucrose (300-400 g/L) system was analyzed by response surface methodology. According to the calculated model in the low sucrose system, maximum predicted levan yield was 47.8 g/L (sucrose 196.8 g/L, ammonium chloride 2.4 g/L, pH 7.0), while in the high sucrose system, levan yield was 99.2 g/L (sucrose 397.6 g/L, ammonium chloride 4.6 g/L, pH 7.4). In addition, protective effect of microbial levan against copper toxicity to Daphnia magna is observed for the first time. The acute toxicity (48 h EC50) of copper decreased from 0.14 to 0.44 mg/L by levan in concentration of 50 ppm.", publisher = "Humana Press Inc, Totowa", journal = "Applied Biochemistry and Biotechnology", title = "High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source", volume = "175", number = "6", pages = "3068-3083", doi = "10.1007/s12010-015-1475-8" }
Kekez, B. D., Gojgić-Cvijović, G. D., Jakovljević, D. M., Stefanović Kojić, J. R., Marković, M. D., Beškoski, V.,& Vrvić, M. M.. (2015). High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. in Applied Biochemistry and Biotechnology Humana Press Inc, Totowa., 175(6), 3068-3083. https://doi.org/10.1007/s12010-015-1475-8
Kekez BD, Gojgić-Cvijović GD, Jakovljević DM, Stefanović Kojić JR, Marković MD, Beškoski V, Vrvić MM. High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. in Applied Biochemistry and Biotechnology. 2015;175(6):3068-3083. doi:10.1007/s12010-015-1475-8 .
Kekez, B. D., Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Stefanović Kojić, Jovana R., Marković, M. D., Beškoski, Vladimir, Vrvić, Miroslav M., "High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source" in Applied Biochemistry and Biotechnology, 175, no. 6 (2015):3068-3083, https://doi.org/10.1007/s12010-015-1475-8 . .