Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls
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Pectin gels and pectin-cellulose binary gels were used as cell wall-mimicking systems to investigate the diffusion ability of a fungal pectin methylesterase. Increasing content of cellulose in the gel appears to result: (i) in longer demethylated blocks thus favouring AaPME processivity, and (ii) in accelerated enzyme kinetics. To better understand this unexpected behaviour, a method was set up to investigate the gel porosity as a function of the cellulose content by following the passive diffusion of three pullulans having different hydrodynamic volumes. Like the enzyme, the pullulans diffused more efficiently in the gels containing the highest proportions of cellulose. Altogether, these results suggest that the gel settled differently during formation according to the respective proportions of the two polysaccharides. With cellulose present, a fraction of pectin would form close interactions with the microfibrils resulting in a larger volume accessible to diffusing molecules. This vo...lume would be related to the cellulose concentration.
Keywords:Pectin / Pectin methylesterase / Enzymatic demethyl-esterification / Degree of blockiness / Pectin-cellulose gels / Gel porosity
Source:Carbohydrate Polymers, 2015, 124, 57-65
- Elsevier Sci Ltd, Oxford