Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls
Само за регистроване кориснике
2015
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Pectin gels and pectin-cellulose binary gels were used as cell wall-mimicking systems to investigate the diffusion ability of a fungal pectin methylesterase. Increasing content of cellulose in the gel appears to result: (i) in longer demethylated blocks thus favouring AaPME processivity, and (ii) in accelerated enzyme kinetics. To better understand this unexpected behaviour, a method was set up to investigate the gel porosity as a function of the cellulose content by following the passive diffusion of three pullulans having different hydrodynamic volumes. Like the enzyme, the pullulans diffused more efficiently in the gels containing the highest proportions of cellulose. Altogether, these results suggest that the gel settled differently during formation according to the respective proportions of the two polysaccharides. With cellulose present, a fraction of pectin would form close interactions with the microfibrils resulting in a larger volume accessible to diffusing molecules. This vo...lume would be related to the cellulose concentration.
Кључне речи:
Pectin / Pectin methylesterase / Enzymatic demethyl-esterification / Degree of blockiness / Pectin-cellulose gels / Gel porosityИзвор:
Carbohydrate Polymers, 2015, 124, 57-65Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-FP7-256716)
- INRA (AIC Enzydam)
DOI: 10.1016/j.carbpol.2015.01.074
ISSN: 0144-8617
PubMed: 25839794
WoS: 000353729400008
Scopus: 2-s2.0-84923544841
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Bonnin, Estelle AU - Mutić, Jelena AU - Nikolić, Jasna AU - Burr, Sally AU - Robert, Paul AU - Crepeau, Marie-Jeanne PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1697 AB - Pectin gels and pectin-cellulose binary gels were used as cell wall-mimicking systems to investigate the diffusion ability of a fungal pectin methylesterase. Increasing content of cellulose in the gel appears to result: (i) in longer demethylated blocks thus favouring AaPME processivity, and (ii) in accelerated enzyme kinetics. To better understand this unexpected behaviour, a method was set up to investigate the gel porosity as a function of the cellulose content by following the passive diffusion of three pullulans having different hydrodynamic volumes. Like the enzyme, the pullulans diffused more efficiently in the gels containing the highest proportions of cellulose. Altogether, these results suggest that the gel settled differently during formation according to the respective proportions of the two polysaccharides. With cellulose present, a fraction of pectin would form close interactions with the microfibrils resulting in a larger volume accessible to diffusing molecules. This volume would be related to the cellulose concentration. PB - Elsevier Sci Ltd, Oxford T2 - Carbohydrate Polymers T1 - Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls VL - 124 SP - 57 EP - 65 DO - 10.1016/j.carbpol.2015.01.074 ER -
@article{ author = "Bonnin, Estelle and Mutić, Jelena and Nikolić, Jasna and Burr, Sally and Robert, Paul and Crepeau, Marie-Jeanne", year = "2015", abstract = "Pectin gels and pectin-cellulose binary gels were used as cell wall-mimicking systems to investigate the diffusion ability of a fungal pectin methylesterase. Increasing content of cellulose in the gel appears to result: (i) in longer demethylated blocks thus favouring AaPME processivity, and (ii) in accelerated enzyme kinetics. To better understand this unexpected behaviour, a method was set up to investigate the gel porosity as a function of the cellulose content by following the passive diffusion of three pullulans having different hydrodynamic volumes. Like the enzyme, the pullulans diffused more efficiently in the gels containing the highest proportions of cellulose. Altogether, these results suggest that the gel settled differently during formation according to the respective proportions of the two polysaccharides. With cellulose present, a fraction of pectin would form close interactions with the microfibrils resulting in a larger volume accessible to diffusing molecules. This volume would be related to the cellulose concentration.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Carbohydrate Polymers", title = "Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls", volume = "124", pages = "57-65", doi = "10.1016/j.carbpol.2015.01.074" }
Bonnin, E., Mutić, J., Nikolić, J., Burr, S., Robert, P.,& Crepeau, M.. (2015). Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls. in Carbohydrate Polymers Elsevier Sci Ltd, Oxford., 124, 57-65. https://doi.org/10.1016/j.carbpol.2015.01.074
Bonnin E, Mutić J, Nikolić J, Burr S, Robert P, Crepeau M. Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls. in Carbohydrate Polymers. 2015;124:57-65. doi:10.1016/j.carbpol.2015.01.074 .
Bonnin, Estelle, Mutić, Jelena, Nikolić, Jasna, Burr, Sally, Robert, Paul, Crepeau, Marie-Jeanne, "Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls" in Carbohydrate Polymers, 124 (2015):57-65, https://doi.org/10.1016/j.carbpol.2015.01.074 . .