DC polarographic examination of Hg2+ reduction applicability to antioxidant activity determination
Само за регистроване кориснике
2015
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Direct current polarographic (DCP) antioxidant assay based on the decrease of anodic limiting current of HydroxoPerhydroxoMercury(II) Complex [Hg(O2H)(OH)] (HPMC) upon addition of antioxidants (AOs) was verified. Here, influence of several common AOs on DCP cathodic currents of both HPMC and Hg2+ was followed. Amperometric titrations of HPMC and Hg2+ with AOs and potentiometric titrations of Hg2+ were performed. The changes of DCP i-E curves and limiting currents decrease during amperometric titrations, as well as end points of amperometric and potentiometric titrations for each AO used as titrant proved the occurrence of Hg2+ reduction, either from complex (HPMC) or salt (HgCl2) solution. Constant H2O2 reduction current during amperometric titration of HPMC further confirmed the participation of Hg2+ as the oxidant species in reaction with AOs. The possibility of determining AO activity of flavonoids, phenolic acids, ascorbic acid and Trolox based on the decrease of Hg2+ reduction cur...rent was demonstrated. Strict correlation (R-2 = 0.96) between a new AO assay named MRAP (Mercury Reduction Antioxidant Power) and HPMC assay was found. Total AO capacity of real samples was measured by the proposed fast, simple and inexpensive MRAP assay as well. (C) 2015 Elsevier Ltd. All rights reserved.
Кључне речи:
antioxidant activity / direct current polarography / hydroxo-perhydroxo mercury(II) complex / phenolicsИзвор:
Electrochimica Acta, 2015, 168, 240-245Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
Финансирање / пројекти:
- Модификације антиоксидативног метаболизма биљака са циљем повећања толеранције на абиотски стрес и идентификација нових биомаркера са применом у ремедијацији и мониторингу деградираних станишта (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
- Утицај сорте и услова гајења на садржај биоактивних компоненти јагодастог и коштичавог воћа и добијање биолошки вредних производа побољшаним и новим технологијама (RS-MESTD-Technological Development (TD or TR)-31093)
DOI: 10.1016/j.electacta.2015.04.008
ISSN: 0013-4686
WoS: 000354055500031
Scopus: 2-s2.0-84927595094
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Suznjevic, Desanka Z. AU - Pastor, Ferenc AU - Gorjanović, Stanislava PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1708 AB - Direct current polarographic (DCP) antioxidant assay based on the decrease of anodic limiting current of HydroxoPerhydroxoMercury(II) Complex [Hg(O2H)(OH)] (HPMC) upon addition of antioxidants (AOs) was verified. Here, influence of several common AOs on DCP cathodic currents of both HPMC and Hg2+ was followed. Amperometric titrations of HPMC and Hg2+ with AOs and potentiometric titrations of Hg2+ were performed. The changes of DCP i-E curves and limiting currents decrease during amperometric titrations, as well as end points of amperometric and potentiometric titrations for each AO used as titrant proved the occurrence of Hg2+ reduction, either from complex (HPMC) or salt (HgCl2) solution. Constant H2O2 reduction current during amperometric titration of HPMC further confirmed the participation of Hg2+ as the oxidant species in reaction with AOs. The possibility of determining AO activity of flavonoids, phenolic acids, ascorbic acid and Trolox based on the decrease of Hg2+ reduction current was demonstrated. Strict correlation (R-2 = 0.96) between a new AO assay named MRAP (Mercury Reduction Antioxidant Power) and HPMC assay was found. Total AO capacity of real samples was measured by the proposed fast, simple and inexpensive MRAP assay as well. (C) 2015 Elsevier Ltd. All rights reserved. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Electrochimica Acta T1 - DC polarographic examination of Hg2+ reduction applicability to antioxidant activity determination VL - 168 SP - 240 EP - 245 DO - 10.1016/j.electacta.2015.04.008 ER -
@article{ author = "Suznjevic, Desanka Z. and Pastor, Ferenc and Gorjanović, Stanislava", year = "2015", abstract = "Direct current polarographic (DCP) antioxidant assay based on the decrease of anodic limiting current of HydroxoPerhydroxoMercury(II) Complex [Hg(O2H)(OH)] (HPMC) upon addition of antioxidants (AOs) was verified. Here, influence of several common AOs on DCP cathodic currents of both HPMC and Hg2+ was followed. Amperometric titrations of HPMC and Hg2+ with AOs and potentiometric titrations of Hg2+ were performed. The changes of DCP i-E curves and limiting currents decrease during amperometric titrations, as well as end points of amperometric and potentiometric titrations for each AO used as titrant proved the occurrence of Hg2+ reduction, either from complex (HPMC) or salt (HgCl2) solution. Constant H2O2 reduction current during amperometric titration of HPMC further confirmed the participation of Hg2+ as the oxidant species in reaction with AOs. The possibility of determining AO activity of flavonoids, phenolic acids, ascorbic acid and Trolox based on the decrease of Hg2+ reduction current was demonstrated. Strict correlation (R-2 = 0.96) between a new AO assay named MRAP (Mercury Reduction Antioxidant Power) and HPMC assay was found. Total AO capacity of real samples was measured by the proposed fast, simple and inexpensive MRAP assay as well. (C) 2015 Elsevier Ltd. All rights reserved.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Electrochimica Acta", title = "DC polarographic examination of Hg2+ reduction applicability to antioxidant activity determination", volume = "168", pages = "240-245", doi = "10.1016/j.electacta.2015.04.008" }
Suznjevic, D. Z., Pastor, F.,& Gorjanović, S.. (2015). DC polarographic examination of Hg2+ reduction applicability to antioxidant activity determination. in Electrochimica Acta Pergamon-Elsevier Science Ltd, Oxford., 168, 240-245. https://doi.org/10.1016/j.electacta.2015.04.008
Suznjevic DZ, Pastor F, Gorjanović S. DC polarographic examination of Hg2+ reduction applicability to antioxidant activity determination. in Electrochimica Acta. 2015;168:240-245. doi:10.1016/j.electacta.2015.04.008 .
Suznjevic, Desanka Z., Pastor, Ferenc, Gorjanović, Stanislava, "DC polarographic examination of Hg2+ reduction applicability to antioxidant activity determination" in Electrochimica Acta, 168 (2015):240-245, https://doi.org/10.1016/j.electacta.2015.04.008 . .