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dc.creatorTamaš, Nenad
dc.creatorDojnov, Biljana
dc.creatorMargetić, Aleksandra
dc.creatorVujčić, Miroslava
dc.creatorSpirović, Bojana
dc.creatorMiletić, Novica
dc.creatorStević, Milan
dc.creatorVujčić, Zoran
dc.date.accessioned2018-11-22T00:31:29Z
dc.date.available2018-11-22T00:31:29Z
dc.date.issued2015
dc.identifier.issn0248-1294
dc.identifier.urihttps://cherry.chem.bg.ac.rs/handle/123456789/1711
dc.description.abstractIntroduction. Aphis pomi (De Geer) has developed resistance to organophosphate and carbamate insecticides, as a result of long-term application of these insecticides in conventional apple orchards. For many years, the only mechanism of resistance identified in aphids was overproduction of insecticide-detoxifying esterases. Materials and methods. Insecticide resistance of A. pomi, collected from two conventional apple orchards (localities of Radmilovac-RA and Bela Crkva-BC) and one organic apple orchard (locality of Surcin-SU), was tested by bioassays and biochemical assays. Results and discussion. Compared with LC50 values for the susceptible population (organic orchard), both populations from the conventional orchards were highly resistant to pirimicarb (234.5 and 52.9 times) and moderately resistant to dimethoate (10.7 and 9.0 times). Increased esterase activity was determined in these two resistant aphid populations. Each of them also produced one esterase isoform more than the susceptible population, when 1-naphthyl acetate was used as a substrate for zymographic detection; when 2-naphthyl acetate was used as a substrate, only one resistant population produced two new esterase isoforms. In one of the resistant populations acetylcholinesterase (AChE) was significantly less inhibited by pirimicarb than in the other resistant population and the susceptible population, which indicates that this population developed another resistance mechanism-Modification of AChE (MACE). Conclusion. Detoxification of insecticides by the metabolic resistance mechanism of esterase enzymes and mechanism of modification of AChE was proven in one aphid population (RA). The other population (BC) has developed only metabolic resistance (enhanced metabolism by esterases), without modification of the insecticide target site (AChE). Development of insecticide resistance was caused by long-term application of acetylcholinesterase inhibitors (organophosphates and carbamates) in these conventional orchards.en
dc.publisherEdp Sciences S A, Les Ulis Cedex A
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.rightsrestrictedAccess
dc.sourceFruits
dc.subjectSerbiaen
dc.subjectappleen
dc.subjectMalus domesticaen
dc.subjectAphis pomien
dc.subjectpesticide resistanceen
dc.subjectpirimicarben
dc.subjectesterasesen
dc.titleResistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae)en
dc.typearticle
dc.rights.licenseARR
dcterms.abstractСпировиц, Бојана; Стевиц, Милан; Дојнов, Биљана; Маргетиц, Aлександра; Вујциц, Мирослава; Вујчић, Зоран; Милетиц, Новица; Тамас, Ненад;
dc.citation.volume70
dc.citation.issue3
dc.citation.spage135
dc.citation.epage142
dc.identifier.wos000354372100003
dc.identifier.doi10.1051/fruits/2015005
dc.citation.other70(3): 135-142
dc.citation.rankM21
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-84928579040


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