Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10
Authorized Users Only
Genčić, Marija S.
Živković, Miodrag V.
Radulović, Niko S.
Article (Published version)
MetadataShow full item record
Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 A degrees C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C-7-C-30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with anoth...er soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production.
Keywords:Streptomyces sp NP10 / Lipidomics / Free fatty acids / Branched cyclopropane fatty acids / Ecophysiological role / Microbial biodiesel production
Source:Applied Microbiology and Biotechnology, 2015, 99, 11, 4815-4833
- Springer, New York
- Combinatorial libraries of heterogeneous catalysts, natural products, and their derivatives and analogues: the way to biologically active compounds (RS-172061)
- Microbial diversity study and characterization of beneficial environmental microorganisms (RS-173048)
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3430