Thrombin stimulates VSMC proliferation through an EGFR-dependent pathway: involvement of MMP-2
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2014
Authors
Smiljanić, Katarina
Obradović, Milan

Jovanovic, Aleksandra

Đorđević, Jelena
Dobutović, Branislava
Jevremovic, Danimir
Marche, Pierre
Isenovic, Esma R.
Article (Published version)

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In this study, the role of epidermal growth factor receptor (EGFR), extracellular signal-regulated kinase (ERK1/2), heparin-binding EGF-like growth factor (HB-EGF), general metalloproteinases, matrix metalloproteinases-2 (MMP-2) in mediating the mitogenic action of thrombin in rat vascular smooth muscle cells (VSMC) was investigated. The incubation of rat VSMC with thrombin (1 U/ml) for 5 min resulted in significant (p lt 0.001) increase of ERK1/2 phosphorylation by 8.7 +/- A 0.9-fold, EGFR phosphorylation by 8.5 +/- A 1.3-fold (p lt 0.001) and DNA synthesis by 3.6 +/- A 0.4-fold (p lt 0.001). Separate 30-min pretreatments with EGFR tyrosine kinase irreversible inhibitor, 10 A mu M PD169540 (PD), and 20 A mu M anti-HB-EGF antibody significantly reduced thrombin-stimulated EGFR and ERK1/2 phosphorylation by 81, 72 % and by 48 and 61 %, respectively. Furthermore, the same pretreatments with PD or anti-HB-EGF antibody reduced thrombin-induced VSMC proliferation by 44 and 45 %, respe...ctively. In addition, 30-min pretreatments with 10 A mu M specific MMP-2 inhibitor significantly reduced thrombin-stimulated phosphorylation of both EGFR and ERK1/2 by 25 %. Moreover, the same pretreatment with MMP-2 inhibitor reduced thrombin-induced VSMC proliferation by 45 %. These results show that the thrombin-induced DNA synthesis correlates with the level of ERK1/2 activation rather than EGFR activation. These results further suggest that thrombin acts through EGFR and ERK 1/2 signaling pathways involving MMP-2 to upregulate proliferation of VSMC.
Keywords:
Thrombin signaling cascade / Pathological VSMC proliferation / MMP-2 / EGFR transactivation / Atherosclerosis / Matrix metalloproteinase / Heparin-binding epidermal growth factor likeSource:
Molecular and Cellular Biochemistry, 2014, 396, 1-2, 147-160Publisher:
- Springer, Dordrecht
Projects:
- Hormonal regulation of expression and activity of the nitric oxide synthase and sodium-potassium pump in experimental models of insulin resistance, diabetes and cardiovascular disorders (RS-173033)
- [337-00-359/2005-01/16]
- Republic of France, Ministry of Foreign Affairs
- CNRS
- University Pierre and Marie Curie
DOI: 10.1007/s11010-014-2151-y
ISSN: 0300-8177
PubMed: 25047892
WoS: 000342437900016
Scopus: 2-s2.0-84907593049
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Hemijski fakultetTY - JOUR AU - Smiljanić, Katarina AU - Obradović, Milan AU - Jovanovic, Aleksandra AU - Đorđević, Jelena AU - Dobutović, Branislava AU - Jevremovic, Danimir AU - Marche, Pierre AU - Isenovic, Esma R. PY - 2014 UR - http://cherry.chem.bg.ac.rs/handle/123456789/1853 AB - In this study, the role of epidermal growth factor receptor (EGFR), extracellular signal-regulated kinase (ERK1/2), heparin-binding EGF-like growth factor (HB-EGF), general metalloproteinases, matrix metalloproteinases-2 (MMP-2) in mediating the mitogenic action of thrombin in rat vascular smooth muscle cells (VSMC) was investigated. The incubation of rat VSMC with thrombin (1 U/ml) for 5 min resulted in significant (p lt 0.001) increase of ERK1/2 phosphorylation by 8.7 +/- A 0.9-fold, EGFR phosphorylation by 8.5 +/- A 1.3-fold (p lt 0.001) and DNA synthesis by 3.6 +/- A 0.4-fold (p lt 0.001). Separate 30-min pretreatments with EGFR tyrosine kinase irreversible inhibitor, 10 A mu M PD169540 (PD), and 20 A mu M anti-HB-EGF antibody significantly reduced thrombin-stimulated EGFR and ERK1/2 phosphorylation by 81, 72 % and by 48 and 61 %, respectively. Furthermore, the same pretreatments with PD or anti-HB-EGF antibody reduced thrombin-induced VSMC proliferation by 44 and 45 %, respectively. In addition, 30-min pretreatments with 10 A mu M specific MMP-2 inhibitor significantly reduced thrombin-stimulated phosphorylation of both EGFR and ERK1/2 by 25 %. Moreover, the same pretreatment with MMP-2 inhibitor reduced thrombin-induced VSMC proliferation by 45 %. These results show that the thrombin-induced DNA synthesis correlates with the level of ERK1/2 activation rather than EGFR activation. These results further suggest that thrombin acts through EGFR and ERK 1/2 signaling pathways involving MMP-2 to upregulate proliferation of VSMC. PB - Springer, Dordrecht T2 - Molecular and Cellular Biochemistry T1 - Thrombin stimulates VSMC proliferation through an EGFR-dependent pathway: involvement of MMP-2 VL - 396 IS - 1-2 SP - 147 EP - 160 DO - 10.1007/s11010-014-2151-y ER -
@article{ author = "Smiljanić, Katarina and Obradović, Milan and Jovanovic, Aleksandra and Đorđević, Jelena and Dobutović, Branislava and Jevremovic, Danimir and Marche, Pierre and Isenovic, Esma R.", year = "2014", url = "http://cherry.chem.bg.ac.rs/handle/123456789/1853", abstract = "In this study, the role of epidermal growth factor receptor (EGFR), extracellular signal-regulated kinase (ERK1/2), heparin-binding EGF-like growth factor (HB-EGF), general metalloproteinases, matrix metalloproteinases-2 (MMP-2) in mediating the mitogenic action of thrombin in rat vascular smooth muscle cells (VSMC) was investigated. The incubation of rat VSMC with thrombin (1 U/ml) for 5 min resulted in significant (p lt 0.001) increase of ERK1/2 phosphorylation by 8.7 +/- A 0.9-fold, EGFR phosphorylation by 8.5 +/- A 1.3-fold (p lt 0.001) and DNA synthesis by 3.6 +/- A 0.4-fold (p lt 0.001). Separate 30-min pretreatments with EGFR tyrosine kinase irreversible inhibitor, 10 A mu M PD169540 (PD), and 20 A mu M anti-HB-EGF antibody significantly reduced thrombin-stimulated EGFR and ERK1/2 phosphorylation by 81, 72 % and by 48 and 61 %, respectively. Furthermore, the same pretreatments with PD or anti-HB-EGF antibody reduced thrombin-induced VSMC proliferation by 44 and 45 %, respectively. In addition, 30-min pretreatments with 10 A mu M specific MMP-2 inhibitor significantly reduced thrombin-stimulated phosphorylation of both EGFR and ERK1/2 by 25 %. Moreover, the same pretreatment with MMP-2 inhibitor reduced thrombin-induced VSMC proliferation by 45 %. These results show that the thrombin-induced DNA synthesis correlates with the level of ERK1/2 activation rather than EGFR activation. These results further suggest that thrombin acts through EGFR and ERK 1/2 signaling pathways involving MMP-2 to upregulate proliferation of VSMC.", publisher = "Springer, Dordrecht", journal = "Molecular and Cellular Biochemistry", title = "Thrombin stimulates VSMC proliferation through an EGFR-dependent pathway: involvement of MMP-2", volume = "396", number = "1-2", pages = "147-160", doi = "10.1007/s11010-014-2151-y" }
Smiljanić K, Obradović M, Jovanovic A, Đorđević J, Dobutović B, Jevremovic D, Marche P, Isenovic ER. Thrombin stimulates VSMC proliferation through an EGFR-dependent pathway: involvement of MMP-2. Molecular and Cellular Biochemistry. 2014;396(1-2):147-160
Smiljanić, K., Obradović, M., Jovanovic, A., Đorđević, J., Dobutović, B., Jevremovic, D., Marche, P.,& Isenovic, E. R. (2014). Thrombin stimulates VSMC proliferation through an EGFR-dependent pathway: involvement of MMP-2. Molecular and Cellular BiochemistrySpringer, Dordrecht., 396(1-2), 147-160. https://doi.org/10.1007/s11010-014-2151-y
Smiljanić Katarina, Obradović Milan, Jovanovic Aleksandra, Đorđević Jelena, Dobutović Branislava, Jevremovic Danimir, Marche Pierre, Isenovic Esma R., "Thrombin stimulates VSMC proliferation through an EGFR-dependent pathway: involvement of MMP-2" 396, no. 1-2 (2014):147-160, https://doi.org/10.1007/s11010-014-2151-y .