A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
Source:
Biomicrofluidics, 2014, 8, 4
Publisher:
Amer Inst Physics, Melville
Funding / projects:
Fulbright Foundation
Harvard MRSEC [DMR-0820484]
RWTH Aachen University
National Science Foundation (NSF) [DMR-1310266]
NSERC PGS D
Excellence Initiative by the German federal government
internal program MEF from the Fraunhofer Gesellschaft [125-600156]
National Science Foundation under NSF Award [ECS-0335765]
TY - JOUR
AU - Ostafe, Raluca
AU - Prodanović, Radivoje
AU - Ung, Lloyd W.
AU - Weitz, David A.
AU - Fischer, Rainer
PY - 2014
UR - http://cherry.chem.bg.ac.rs/handle/123456789/1873
AB - A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
PB - Amer Inst Physics, Melville
T2 - Biomicrofluidics
T1 - A high-throughput cellulase screening system based on droplet microfluidics
VL - 8
IS - 4
DO - 10.1063/1.4886771
UR - Kon_2756
ER -
@article{
author = "Ostafe, Raluca and Prodanović, Radivoje and Ung, Lloyd W. and Weitz, David A. and Fischer, Rainer",
year = "2014",
abstract = "A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.",
publisher = "Amer Inst Physics, Melville",
journal = "Biomicrofluidics",
title = "A high-throughput cellulase screening system based on droplet microfluidics",
volume = "8",
number = "4",
doi = "10.1063/1.4886771",
url = "Kon_2756"
}
Ostafe, R., Prodanović, R., Ung, L. W., Weitz, D. A.,& Fischer, R.. (2014). A high-throughput cellulase screening system based on droplet microfluidics. in Biomicrofluidics
Amer Inst Physics, Melville., 8(4).
https://doi.org/10.1063/1.4886771
Kon_2756
Ostafe R, Prodanović R, Ung LW, Weitz DA, Fischer R. A high-throughput cellulase screening system based on droplet microfluidics. in Biomicrofluidics. 2014;8(4).
doi:10.1063/1.4886771
Kon_2756 .
Ostafe, Raluca, Prodanović, Radivoje, Ung, Lloyd W., Weitz, David A., Fischer, Rainer, "A high-throughput cellulase screening system based on droplet microfluidics" in Biomicrofluidics, 8, no. 4 (2014),
https://doi.org/10.1063/1.4886771 .,
Kon_2756 .
