A high-throughput cellulase screening system based on droplet microfluidics
Апстракт
A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
Извор:
Biomicrofluidics, 2014, 8, 4Издавач:
- Amer Inst Physics, Melville
Финансирање / пројекти:
- Fulbright Foundation
- Harvard MRSEC [DMR-0820484]
- RWTH Aachen University
- National Science Foundation (NSF) [DMR-1310266]
- NSERC PGS D
- Excellence Initiative by the German federal government
- internal program MEF from the Fraunhofer Gesellschaft [125-600156]
- National Science Foundation under NSF Award [ECS-0335765]
Напомена:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3666
DOI: 10.1063/1.4886771
ISSN: 1932-1058
PubMed: 25379082
WoS: 000344225400002
Scopus: 2-s2.0-84919896676
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Ostafe, Raluca AU - Prodanović, Radivoje AU - Ung, Lloyd W. AU - Weitz, David A. AU - Fischer, Rainer PY - 2014 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1873 AB - A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License. PB - Amer Inst Physics, Melville T2 - Biomicrofluidics T1 - A high-throughput cellulase screening system based on droplet microfluidics VL - 8 IS - 4 DO - 10.1063/1.4886771 ER -
@article{ author = "Ostafe, Raluca and Prodanović, Radivoje and Ung, Lloyd W. and Weitz, David A. and Fischer, Rainer", year = "2014", abstract = "A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.", publisher = "Amer Inst Physics, Melville", journal = "Biomicrofluidics", title = "A high-throughput cellulase screening system based on droplet microfluidics", volume = "8", number = "4", doi = "10.1063/1.4886771" }
Ostafe, R., Prodanović, R., Ung, L. W., Weitz, D. A.,& Fischer, R.. (2014). A high-throughput cellulase screening system based on droplet microfluidics. in Biomicrofluidics Amer Inst Physics, Melville., 8(4). https://doi.org/10.1063/1.4886771
Ostafe R, Prodanović R, Ung LW, Weitz DA, Fischer R. A high-throughput cellulase screening system based on droplet microfluidics. in Biomicrofluidics. 2014;8(4). doi:10.1063/1.4886771 .
Ostafe, Raluca, Prodanović, Radivoje, Ung, Lloyd W., Weitz, David A., Fischer, Rainer, "A high-throughput cellulase screening system based on droplet microfluidics" in Biomicrofluidics, 8, no. 4 (2014), https://doi.org/10.1063/1.4886771 . .