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A high-throughput cellulase screening system based on droplet microfluidics
dc.creator | Ostafe, Raluca | |
dc.creator | Prodanović, Radivoje | |
dc.creator | Ung, Lloyd W. | |
dc.creator | Weitz, David A. | |
dc.creator | Fischer, Rainer | |
dc.date.accessioned | 2018-11-22T00:29:30Z | |
dc.date.available | 2018-11-22T00:29:30Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1932-1058 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/1873 | |
dc.description.abstract | A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License. | en |
dc.publisher | Amer Inst Physics, Melville | |
dc.relation | Fulbright Foundation | |
dc.relation | Harvard MRSEC [DMR-0820484] | |
dc.relation | RWTH Aachen University | |
dc.relation | National Science Foundation (NSF) [DMR-1310266] | |
dc.relation | NSERC PGS D | |
dc.relation | Excellence Initiative by the German federal government | |
dc.relation | internal program MEF from the Fraunhofer Gesellschaft [125-600156] | |
dc.relation | National Science Foundation under NSF Award [ECS-0335765] | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Biomicrofluidics | |
dc.title | A high-throughput cellulase screening system based on droplet microfluidics | en |
dc.type | article | |
dc.rights.license | BY | |
dcterms.abstract | Wеитз, Давид A.; Унг, W. Ллоyд; Фисцхер, Раинер; Продановић, Радивоје; Остафе, Ралуца; | |
dc.citation.volume | 8 | |
dc.citation.issue | 4 | |
dc.identifier.wos | 000344225400002 | |
dc.identifier.doi | 10.1063/1.4886771 | |
dc.citation.other | 8(4): | |
dc.citation.rank | aM21 | |
dc.identifier.pmid | 25379082 | |
dc.description.other | Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3666] | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-84919896676 | |
dc.identifier.fulltext | https://cherry.chem.bg.ac.rs/bitstream/id/8924/1871.pdf |