The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols
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2015
Authors
Krstić-Ristivojević, MajaStojadinović, Marija M.
Smiljanić, Katarina
Stanić-Vučinić, Dragana
Ćirković-Veličković, Tanja
Article (Published version)
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It has been shown before that dietary polyphenols possess cancer chemopreventive effects. As cervical cancer is the second leading genital malignancy in women after breast cancer, the anti-cervical cancer effects of polyphenol extracts of commonly used beverages (green tea, coffee and cocoa) were tested and compared in HeLa cells. All the extracts induced apoptosis of HeLa cells, but green tea was the most potent. However, as opposed to green tea which induced a strong anti-proliferative response in HeLa cells, coffee and cocoa extracts promoted the proliferation of surviving cells. After short-term exposure, green tea and coffee extracts, but not cocoa, induced the formation of intracellular reactive oxygen species. Only the green tea extract increased the production of superoxide anion radicals and decreased reduced glutathione levels. Gene expression of Cu/Zn and Mn-superoxide dismutase or catalase was unaltered in cells treated with extracts, but green tea partially inhibited catal...ase activity. The cytotoxic activity of green tea and coffee extracts was partially inhibited by vitamin C. The in vitro anti-cervical cancer potency of tested polyphenol extracts is related to their pro-oxidant and anti-proliferative activities and are exhibited in the following order: green tea gt coffee gt cocoa, with only green tea showing both pro-oxidative and anti-proliferative action.
Source:
RSC Advances, 2015, 5, 5, 3260-3268Publisher:
- Royal Soc Chemistry, Cambridge
Funding / projects:
- Molecular properties and modifications of some respiratory and nutritional allergens (RS-172024)
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-256716)
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3449
DOI: 10.1039/c4ra13230k
ISSN: 2046-2069
WoS: 000346405200019
Scopus: 2-s2.0-84918799282
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Krstić-Ristivojević, Maja AU - Stojadinović, Marija M. AU - Smiljanić, Katarina AU - Stanić-Vučinić, Dragana AU - Ćirković-Veličković, Tanja PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1888 AB - It has been shown before that dietary polyphenols possess cancer chemopreventive effects. As cervical cancer is the second leading genital malignancy in women after breast cancer, the anti-cervical cancer effects of polyphenol extracts of commonly used beverages (green tea, coffee and cocoa) were tested and compared in HeLa cells. All the extracts induced apoptosis of HeLa cells, but green tea was the most potent. However, as opposed to green tea which induced a strong anti-proliferative response in HeLa cells, coffee and cocoa extracts promoted the proliferation of surviving cells. After short-term exposure, green tea and coffee extracts, but not cocoa, induced the formation of intracellular reactive oxygen species. Only the green tea extract increased the production of superoxide anion radicals and decreased reduced glutathione levels. Gene expression of Cu/Zn and Mn-superoxide dismutase or catalase was unaltered in cells treated with extracts, but green tea partially inhibited catalase activity. The cytotoxic activity of green tea and coffee extracts was partially inhibited by vitamin C. The in vitro anti-cervical cancer potency of tested polyphenol extracts is related to their pro-oxidant and anti-proliferative activities and are exhibited in the following order: green tea gt coffee gt cocoa, with only green tea showing both pro-oxidative and anti-proliferative action. PB - Royal Soc Chemistry, Cambridge T2 - RSC Advances T1 - The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols VL - 5 IS - 5 SP - 3260 EP - 3268 DO - 10.1039/c4ra13230k ER -
@article{ author = "Krstić-Ristivojević, Maja and Stojadinović, Marija M. and Smiljanić, Katarina and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja", year = "2015", abstract = "It has been shown before that dietary polyphenols possess cancer chemopreventive effects. As cervical cancer is the second leading genital malignancy in women after breast cancer, the anti-cervical cancer effects of polyphenol extracts of commonly used beverages (green tea, coffee and cocoa) were tested and compared in HeLa cells. All the extracts induced apoptosis of HeLa cells, but green tea was the most potent. However, as opposed to green tea which induced a strong anti-proliferative response in HeLa cells, coffee and cocoa extracts promoted the proliferation of surviving cells. After short-term exposure, green tea and coffee extracts, but not cocoa, induced the formation of intracellular reactive oxygen species. Only the green tea extract increased the production of superoxide anion radicals and decreased reduced glutathione levels. Gene expression of Cu/Zn and Mn-superoxide dismutase or catalase was unaltered in cells treated with extracts, but green tea partially inhibited catalase activity. The cytotoxic activity of green tea and coffee extracts was partially inhibited by vitamin C. The in vitro anti-cervical cancer potency of tested polyphenol extracts is related to their pro-oxidant and anti-proliferative activities and are exhibited in the following order: green tea gt coffee gt cocoa, with only green tea showing both pro-oxidative and anti-proliferative action.", publisher = "Royal Soc Chemistry, Cambridge", journal = "RSC Advances", title = "The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols", volume = "5", number = "5", pages = "3260-3268", doi = "10.1039/c4ra13230k" }
Krstić-Ristivojević, M., Stojadinović, M. M., Smiljanić, K., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2015). The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols. in RSC Advances Royal Soc Chemistry, Cambridge., 5(5), 3260-3268. https://doi.org/10.1039/c4ra13230k
Krstić-Ristivojević M, Stojadinović MM, Smiljanić K, Stanić-Vučinić D, Ćirković-Veličković T. The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols. in RSC Advances. 2015;5(5):3260-3268. doi:10.1039/c4ra13230k .
Krstić-Ristivojević, Maja, Stojadinović, Marija M., Smiljanić, Katarina, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols" in RSC Advances, 5, no. 5 (2015):3260-3268, https://doi.org/10.1039/c4ra13230k . .