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dc.creatorRašković, Brankica
dc.creatorBozovic, Olga
dc.creatorProdanović, Radivoje
dc.creatorNiketic, Vesna
dc.creatorPolović, Natalija
dc.date.accessioned2018-11-22T00:29:50Z
dc.date.available2018-11-22T00:29:50Z
dc.date.issued2014
dc.identifier.issn1389-1723
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1902
dc.description.abstractA novel collagenolytic serine protease was identified and then purified (along with ficin) to apparent homogeneity from the latex of fig (Ficus carica, var. Brown Turkey) by two step chromatographic procedure using gel and covalent chromatography. The enzyme is a monomeric protein of molecular mass of 41 +/- 9 kDa as estimated by analytical gel filtration chromatography. It is an acidic protein with a pI value of approximately 5 and optimal activity at pH 8.0-8.5 and temperature 60 degrees C. The enzymatic activity was strongly inhibited by PMSF and Pefabloc SC, indicating that the enzyme is a serine protease. The enzyme showed specificity towards gelatin and collagen (215 GDU/mg and 24.8 CDU/mg, respectively) and non-specific protease activity (0.18 U/mg against casein). The enzyme was stable and retained full activity over a broad range of pH and temperature. The fig latex collagenolytic protease is potentially useful as a non-microbial enzyme with collagenolytic activity for various applications in the fields of biochemistry, biotechnology and medicine. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.en
dc.publisherSoc Bioscience Bioengineering Japan, Osaka
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Bioscience and Bioengineering
dc.subjectCollagenolytic activityen
dc.subjectGelatinolytic activityen
dc.subjectSerine proteaseen
dc.subjectFig latexen
dc.subjectEnzyme purificationen
dc.titleIdentification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latexen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПоловић, Наталија; Расковиц, Бранкица; Никетиц, Весна; Продановић, Радивоје; Бозовиц, Олга;
dc.citation.volume118
dc.citation.issue6
dc.citation.spage622
dc.citation.epage627
dc.identifier.wos000347869100003
dc.identifier.doi10.1016/j.jbiosc.2014.05.020
dc.citation.other118(6): 622-627
dc.citation.rankM22
dc.identifier.pmid24982021
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84922819521
dc.identifier.rcubKon_2785


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