Essential oil of Carum copticum seeds, obtained from a local shop, was extracted and content of thymol was analyzed using square-wave voltammetry at boron-doped diamond electrode. The effect of various parameters, such as pH of supporting electrolyte and square-wave voltammetric parameters (modulation amplitude and frequency), was examined. In Britton-Robinson buffer solution (pH 4), thymol provided a single and oval-shaped irreversible oxidation peak at +1.13 V versus silver/silver chloride potassium electrode (3 M). Under optimal experimental conditions, a plot of peak height against concentration of thymol was found to be linear over the range of 4 to 100 mu M consisting of two linear ranges: from 4 to 20 mu M (R-2 = 0.9964) and from 20 to 100 mu M (R-2 = 0.9993). The effect of potential interferences such as p-cymene and gamma-terpinene (major components in essential oil of C copticum seeds) was evaluated. Thus, the proposed method displays a sufficient selectivity toward thymol wi...th a detection limit of 3.9 mu M, and it was successfully applied for the determination of thymol in essential oil of C copticum seeds. The Prussian blue method was used for validation of the proposed electroanalytical method.
TY - JOUR
AU - Stanković, Dalibor
PY - 2015
UR - https://cherry.chem.bg.ac.rs/handle/123456789/1952
AB - Essential oil of Carum copticum seeds, obtained from a local shop, was extracted and content of thymol was analyzed using square-wave voltammetry at boron-doped diamond electrode. The effect of various parameters, such as pH of supporting electrolyte and square-wave voltammetric parameters (modulation amplitude and frequency), was examined. In Britton-Robinson buffer solution (pH 4), thymol provided a single and oval-shaped irreversible oxidation peak at +1.13 V versus silver/silver chloride potassium electrode (3 M). Under optimal experimental conditions, a plot of peak height against concentration of thymol was found to be linear over the range of 4 to 100 mu M consisting of two linear ranges: from 4 to 20 mu M (R-2 = 0.9964) and from 20 to 100 mu M (R-2 = 0.9993). The effect of potential interferences such as p-cymene and gamma-terpinene (major components in essential oil of C copticum seeds) was evaluated. Thus, the proposed method displays a sufficient selectivity toward thymol with a detection limit of 3.9 mu M, and it was successfully applied for the determination of thymol in essential oil of C copticum seeds. The Prussian blue method was used for validation of the proposed electroanalytical method.
PB - Academic Press Inc Elsevier Science, San Diego
T2 - Analytical Biochemistry
T1 - Sensitive voltammetric determination of thymol in essential oil of Carum copticum seeds using boron-doped diamond electrode
VL - 486
SP - 1
EP - 4
DO - 10.1016/j.ab.2015.06.026
UR - Kon_2907
ER -
@article{
author = "Stanković, Dalibor",
year = "2015",
abstract = "Essential oil of Carum copticum seeds, obtained from a local shop, was extracted and content of thymol was analyzed using square-wave voltammetry at boron-doped diamond electrode. The effect of various parameters, such as pH of supporting electrolyte and square-wave voltammetric parameters (modulation amplitude and frequency), was examined. In Britton-Robinson buffer solution (pH 4), thymol provided a single and oval-shaped irreversible oxidation peak at +1.13 V versus silver/silver chloride potassium electrode (3 M). Under optimal experimental conditions, a plot of peak height against concentration of thymol was found to be linear over the range of 4 to 100 mu M consisting of two linear ranges: from 4 to 20 mu M (R-2 = 0.9964) and from 20 to 100 mu M (R-2 = 0.9993). The effect of potential interferences such as p-cymene and gamma-terpinene (major components in essential oil of C copticum seeds) was evaluated. Thus, the proposed method displays a sufficient selectivity toward thymol with a detection limit of 3.9 mu M, and it was successfully applied for the determination of thymol in essential oil of C copticum seeds. The Prussian blue method was used for validation of the proposed electroanalytical method.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Analytical Biochemistry",
title = "Sensitive voltammetric determination of thymol in essential oil of Carum copticum seeds using boron-doped diamond electrode",
volume = "486",
pages = "1-4",
doi = "10.1016/j.ab.2015.06.026",
url = "Kon_2907"
}
Stanković, D.. (2015). Sensitive voltammetric determination of thymol in essential oil of Carum copticum seeds using boron-doped diamond electrode. in Analytical Biochemistry
Academic Press Inc Elsevier Science, San Diego., 486, 1-4.
https://doi.org/10.1016/j.ab.2015.06.026
Kon_2907
Stanković D. Sensitive voltammetric determination of thymol in essential oil of Carum copticum seeds using boron-doped diamond electrode. in Analytical Biochemistry. 2015;486:1-4.
doi:10.1016/j.ab.2015.06.026
Kon_2907 .
Stanković, Dalibor, "Sensitive voltammetric determination of thymol in essential oil of Carum copticum seeds using boron-doped diamond electrode" in Analytical Biochemistry, 486 (2015):1-4,
https://doi.org/10.1016/j.ab.2015.06.026 .,
Kon_2907 .
