In this study we proposed electrochemical behavior and determination of baicalein, main active flavonoid from Scutellaria baicalensis, using boron doped diamond electrode. In Britton-Robinson buffer solution, pH 2, baicalein provides two single oval shape oxidation peaks at +0.5 V/Ag-AgCl and a second one at +0.8 V/Ag-AgCl. For quantification of baicalein square wave voltammetry was used. Under optimized parameters calibration curve was linear over the range 1-95 mu M with detection limit of 0.26 mu M. After eight successful measurements repeatability was 3.7%. Proposed method was applied for determination of baicalein in real samples with excellent recovery. The influence of the most common interferences, biomolecules presented in urine was investigated. The developed approach could be beneficial in analysis of widely used flavonoid in biological samples. (C) 2015 Elsevier B.V. All rights reserved.
TY - JOUR
AU - Kuzmanović, Darko
AU - Stanković, Dalibor
AU - Manojlović, Dragan D.
AU - Kalcher, Kurt
AU - Roglić, Goran
PY - 2015
UR - http://cherry.chem.bg.ac.rs/handle/123456789/1973
AB - In this study we proposed electrochemical behavior and determination of baicalein, main active flavonoid from Scutellaria baicalensis, using boron doped diamond electrode. In Britton-Robinson buffer solution, pH 2, baicalein provides two single oval shape oxidation peaks at +0.5 V/Ag-AgCl and a second one at +0.8 V/Ag-AgCl. For quantification of baicalein square wave voltammetry was used. Under optimized parameters calibration curve was linear over the range 1-95 mu M with detection limit of 0.26 mu M. After eight successful measurements repeatability was 3.7%. Proposed method was applied for determination of baicalein in real samples with excellent recovery. The influence of the most common interferences, biomolecules presented in urine was investigated. The developed approach could be beneficial in analysis of widely used flavonoid in biological samples. (C) 2015 Elsevier B.V. All rights reserved.
PB - Elsevier Science Sa, Lausanne
T2 - Diamond and Related Materials
T1 - Baicalein - main active flavonoid from Scutellaria baicalensis - voltammetric sensing in human samples using boron doped diamond electrode
VL - 58
SP - 35
EP - 39
DO - 10.1016/j.diamond.2015.06.002
ER -
@article{
author = "Kuzmanović, Darko and Stanković, Dalibor and Manojlović, Dragan D. and Kalcher, Kurt and Roglić, Goran",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1973",
abstract = "In this study we proposed electrochemical behavior and determination of baicalein, main active flavonoid from Scutellaria baicalensis, using boron doped diamond electrode. In Britton-Robinson buffer solution, pH 2, baicalein provides two single oval shape oxidation peaks at +0.5 V/Ag-AgCl and a second one at +0.8 V/Ag-AgCl. For quantification of baicalein square wave voltammetry was used. Under optimized parameters calibration curve was linear over the range 1-95 mu M with detection limit of 0.26 mu M. After eight successful measurements repeatability was 3.7%. Proposed method was applied for determination of baicalein in real samples with excellent recovery. The influence of the most common interferences, biomolecules presented in urine was investigated. The developed approach could be beneficial in analysis of widely used flavonoid in biological samples. (C) 2015 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Diamond and Related Materials",
title = "Baicalein - main active flavonoid from Scutellaria baicalensis - voltammetric sensing in human samples using boron doped diamond electrode",
volume = "58",
pages = "35-39",
doi = "10.1016/j.diamond.2015.06.002"
}
Kuzmanović, D., Stanković, D., Manojlović, D. D., Kalcher, K.,& Roglić, G. (2015). Baicalein - main active flavonoid from Scutellaria baicalensis - voltammetric sensing in human samples using boron doped diamond electrode.
Diamond and Related Materials
Elsevier Science Sa, Lausanne., 58, 35-39.
https://doi.org/10.1016/j.diamond.2015.06.002
Kuzmanović D, Stanković D, Manojlović DD, Kalcher K, Roglić G. Baicalein - main active flavonoid from Scutellaria baicalensis - voltammetric sensing in human samples using boron doped diamond electrode. Diamond and Related Materials. 2015;58:35-39
Kuzmanović Darko, Stanković Dalibor, Manojlović Dragan D., Kalcher Kurt, Roglić Goran, "Baicalein - main active flavonoid from Scutellaria baicalensis - voltammetric sensing in human samples using boron doped diamond electrode" Diamond and Related Materials, 58 (2015):35-39,
https://doi.org/10.1016/j.diamond.2015.06.002 .
