With the purpose of finding a suitable available inducer in combination with starvation, carbohydrate mixtures from triticale were used and compared with well-known amylase inducers in fungi. Carbohydrate mixtures from triticale induced the production of an amylase cocktail (alpha-amylase and glucoamylase) in Aspergillus niger, unlike induction with well-known inducers that induce only glucoamylase, shown by zymography and TLC analysis of the carbohydrate mixtures before and after fermentation. Glucoamylase production by A. niger was the highest in the presence of the extract obtained after auto-hydrolysis of starch from triticale (95.88 U mL(-1)). Carbohydrate mixtures from triticale induced the production of alpha-amylase in A. oryzae. More alpha-amylase isoforms were detected when using a complex carbohydrate mixture, compared to induction with maltose or starch. A 48-h induction was the most efficient using a triticale extract (101.35 U mL(-1)). Carbohydrates from triticale extract...s could be used as very good cheap amylase inducers. Triticale, still not fully utilized, could be taken into consideration as an inducer in amylase production by Aspergillus sp, and in such a way, it could be used as the sole substrate in fermentation.
TY - JOUR
AU - Dojnov, Biljana
AU - Grujić, Marica
AU - Percevic, Bojana
AU - Vujčić, Zoran
PY - 2015
UR - https://cherry.chem.bg.ac.rs/handle/123456789/1993
AB - With the purpose of finding a suitable available inducer in combination with starvation, carbohydrate mixtures from triticale were used and compared with well-known amylase inducers in fungi. Carbohydrate mixtures from triticale induced the production of an amylase cocktail (alpha-amylase and glucoamylase) in Aspergillus niger, unlike induction with well-known inducers that induce only glucoamylase, shown by zymography and TLC analysis of the carbohydrate mixtures before and after fermentation. Glucoamylase production by A. niger was the highest in the presence of the extract obtained after auto-hydrolysis of starch from triticale (95.88 U mL(-1)). Carbohydrate mixtures from triticale induced the production of alpha-amylase in A. oryzae. More alpha-amylase isoforms were detected when using a complex carbohydrate mixture, compared to induction with maltose or starch. A 48-h induction was the most efficient using a triticale extract (101.35 U mL(-1)). Carbohydrates from triticale extracts could be used as very good cheap amylase inducers. Triticale, still not fully utilized, could be taken into consideration as an inducer in amylase production by Aspergillus sp, and in such a way, it could be used as the sole substrate in fermentation.
PB - Serbian Chemical Soc, Belgrade
T2 - Journal of the Serbian Chemical Society
T1 - Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale
VL - 80
IS - 10
SP - 1279
EP - 1288
DO - 10.2298/JSC150317043D
ER -
@article{
author = "Dojnov, Biljana and Grujić, Marica and Percevic, Bojana and Vujčić, Zoran",
year = "2015",
abstract = "With the purpose of finding a suitable available inducer in combination with starvation, carbohydrate mixtures from triticale were used and compared with well-known amylase inducers in fungi. Carbohydrate mixtures from triticale induced the production of an amylase cocktail (alpha-amylase and glucoamylase) in Aspergillus niger, unlike induction with well-known inducers that induce only glucoamylase, shown by zymography and TLC analysis of the carbohydrate mixtures before and after fermentation. Glucoamylase production by A. niger was the highest in the presence of the extract obtained after auto-hydrolysis of starch from triticale (95.88 U mL(-1)). Carbohydrate mixtures from triticale induced the production of alpha-amylase in A. oryzae. More alpha-amylase isoforms were detected when using a complex carbohydrate mixture, compared to induction with maltose or starch. A 48-h induction was the most efficient using a triticale extract (101.35 U mL(-1)). Carbohydrates from triticale extracts could be used as very good cheap amylase inducers. Triticale, still not fully utilized, could be taken into consideration as an inducer in amylase production by Aspergillus sp, and in such a way, it could be used as the sole substrate in fermentation.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale",
volume = "80",
number = "10",
pages = "1279-1288",
doi = "10.2298/JSC150317043D"
}
Dojnov, B., Grujić, M., Percevic, B.,& Vujčić, Z.. (2015). Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 80(10), 1279-1288.
https://doi.org/10.2298/JSC150317043D
Dojnov B, Grujić M, Percevic B, Vujčić Z. Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale. in Journal of the Serbian Chemical Society. 2015;80(10):1279-1288.
doi:10.2298/JSC150317043D .
Dojnov, Biljana, Grujić, Marica, Percevic, Bojana, Vujčić, Zoran, "Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale" in Journal of the Serbian Chemical Society, 80, no. 10 (2015):1279-1288,
https://doi.org/10.2298/JSC150317043D . .
