A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles
Samo za registrovane korisnike
2015
Autori
Habtamu, Henok B.Sentić, Milica
Silvestrini, Morena
De Leo, Luigina
Not, Tarcisio
Arbault, Stephane
Manojlović, Dragan D.
Šojić, Nešo
Ugo, Paolo
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
We report here the design of a novel immunosensor and its application for celiac disease diagnosis, based on an electrogenerated chemiluminescence (ECL) readout, using membrane-templated gold nanoelectrode ensembles (NEEs) as a detection platform. An original sensing strategy is presented by segregating spatially the initial electrochemical reaction and the location of the immobilized biomolecules where ECL is finally emitted. The recognition scaffold is the following: tissue transglutaminase (tTG) is immobilized as a capturing agent on the polycarbonate (PC) surface of the track-etched templating membrane. It captures the target tissue transglutaminase antibody (anti-tTG), and finally allows the immobilization of a streptavidin-modified ruthenium-based ECL label via reaction with a suitable biotinylated secondary antibody. The application of an oxidizing potential in a tri-n-propylamine (TPrA) solution generates an intense and sharp ECL signal, suitable for analytical purposes. Voltam...metric and ECL analyses evidenced that the ruthenium complex is not oxidized directly at the surface of the nanoelectrodes; instead ECL is generated following the TPrA oxidation, which produces the TPrA(center dot+) and TPrA(center dot) radicals. With NEEs operating under total overlap diffusion conditions, high local fluxes of these reactive radicals are produced by the nanoelectrodes in the immediate vicinity of the ECL labels, so that they efficiently generate the ECL signal. The radicals can diffuse over short distances and react with the Ru(bpy)(3)(2+) label. In addition, the ECL emission is obtained by applying a potential of 0.88 V versus Ag/AgCl, which is about 0.3 V lower than when ECL is initiated by the electrochemical oxidation of Ru(bpy). The immunosensor provides ECL signals which scale with anti-tTG concentration with a linearity range between 1.5 ng.mL(-1) and 10 mu g.mL(-1) and a detection limit of 0.5 ng.mL(-1). The sensor is finally applied to the analysis of anti-tTG in human serum samples, showing to be suitable to discriminate between healthy and celiac patients.
Izvor:
Analytical Chemistry, 2015, 87, 24, 12080-12087Izdavač:
- Amer Chemical Soc, Washington
Finansiranje / projekti:
- French Foreign Ministry (Bourse dExcellence Eiffel)
- Ministry of Science and Technological Development (Republic of Serbia)
- Cross-Border Cooperation Italy-Slovenia Program - Strategic Project TRANS2CARE
- MIUR (Rome) [PRIN 2010AXENJ8]
- Erasmus Placement Program
Napomena:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3329
DOI: 10.1021/acs.analchem.5b02801
ISSN: 0003-2700
PubMed: 26556023
WoS: 000366871500021
Scopus: 2-s2.0-84950139730
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Habtamu, Henok B. AU - Sentić, Milica AU - Silvestrini, Morena AU - De Leo, Luigina AU - Not, Tarcisio AU - Arbault, Stephane AU - Manojlović, Dragan D. AU - Šojić, Nešo AU - Ugo, Paolo PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2014 AB - We report here the design of a novel immunosensor and its application for celiac disease diagnosis, based on an electrogenerated chemiluminescence (ECL) readout, using membrane-templated gold nanoelectrode ensembles (NEEs) as a detection platform. An original sensing strategy is presented by segregating spatially the initial electrochemical reaction and the location of the immobilized biomolecules where ECL is finally emitted. The recognition scaffold is the following: tissue transglutaminase (tTG) is immobilized as a capturing agent on the polycarbonate (PC) surface of the track-etched templating membrane. It captures the target tissue transglutaminase antibody (anti-tTG), and finally allows the immobilization of a streptavidin-modified ruthenium-based ECL label via reaction with a suitable biotinylated secondary antibody. The application of an oxidizing potential in a tri-n-propylamine (TPrA) solution generates an intense and sharp ECL signal, suitable for analytical purposes. Voltammetric and ECL analyses evidenced that the ruthenium complex is not oxidized directly at the surface of the nanoelectrodes; instead ECL is generated following the TPrA oxidation, which produces the TPrA(center dot+) and TPrA(center dot) radicals. With NEEs operating under total overlap diffusion conditions, high local fluxes of these reactive radicals are produced by the nanoelectrodes in the immediate vicinity of the ECL labels, so that they efficiently generate the ECL signal. The radicals can diffuse over short distances and react with the Ru(bpy)(3)(2+) label. In addition, the ECL emission is obtained by applying a potential of 0.88 V versus Ag/AgCl, which is about 0.3 V lower than when ECL is initiated by the electrochemical oxidation of Ru(bpy). The immunosensor provides ECL signals which scale with anti-tTG concentration with a linearity range between 1.5 ng.mL(-1) and 10 mu g.mL(-1) and a detection limit of 0.5 ng.mL(-1). The sensor is finally applied to the analysis of anti-tTG in human serum samples, showing to be suitable to discriminate between healthy and celiac patients. PB - Amer Chemical Soc, Washington T2 - Analytical Chemistry T1 - A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles VL - 87 IS - 24 SP - 12080 EP - 12087 DO - 10.1021/acs.analchem.5b02801 ER -
@article{ author = "Habtamu, Henok B. and Sentić, Milica and Silvestrini, Morena and De Leo, Luigina and Not, Tarcisio and Arbault, Stephane and Manojlović, Dragan D. and Šojić, Nešo and Ugo, Paolo", year = "2015", abstract = "We report here the design of a novel immunosensor and its application for celiac disease diagnosis, based on an electrogenerated chemiluminescence (ECL) readout, using membrane-templated gold nanoelectrode ensembles (NEEs) as a detection platform. An original sensing strategy is presented by segregating spatially the initial electrochemical reaction and the location of the immobilized biomolecules where ECL is finally emitted. The recognition scaffold is the following: tissue transglutaminase (tTG) is immobilized as a capturing agent on the polycarbonate (PC) surface of the track-etched templating membrane. It captures the target tissue transglutaminase antibody (anti-tTG), and finally allows the immobilization of a streptavidin-modified ruthenium-based ECL label via reaction with a suitable biotinylated secondary antibody. The application of an oxidizing potential in a tri-n-propylamine (TPrA) solution generates an intense and sharp ECL signal, suitable for analytical purposes. Voltammetric and ECL analyses evidenced that the ruthenium complex is not oxidized directly at the surface of the nanoelectrodes; instead ECL is generated following the TPrA oxidation, which produces the TPrA(center dot+) and TPrA(center dot) radicals. With NEEs operating under total overlap diffusion conditions, high local fluxes of these reactive radicals are produced by the nanoelectrodes in the immediate vicinity of the ECL labels, so that they efficiently generate the ECL signal. The radicals can diffuse over short distances and react with the Ru(bpy)(3)(2+) label. In addition, the ECL emission is obtained by applying a potential of 0.88 V versus Ag/AgCl, which is about 0.3 V lower than when ECL is initiated by the electrochemical oxidation of Ru(bpy). The immunosensor provides ECL signals which scale with anti-tTG concentration with a linearity range between 1.5 ng.mL(-1) and 10 mu g.mL(-1) and a detection limit of 0.5 ng.mL(-1). The sensor is finally applied to the analysis of anti-tTG in human serum samples, showing to be suitable to discriminate between healthy and celiac patients.", publisher = "Amer Chemical Soc, Washington", journal = "Analytical Chemistry", title = "A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles", volume = "87", number = "24", pages = "12080-12087", doi = "10.1021/acs.analchem.5b02801" }
Habtamu, H. B., Sentić, M., Silvestrini, M., De Leo, L., Not, T., Arbault, S., Manojlović, D. D., Šojić, N.,& Ugo, P.. (2015). A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. in Analytical Chemistry Amer Chemical Soc, Washington., 87(24), 12080-12087. https://doi.org/10.1021/acs.analchem.5b02801
Habtamu HB, Sentić M, Silvestrini M, De Leo L, Not T, Arbault S, Manojlović DD, Šojić N, Ugo P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. in Analytical Chemistry. 2015;87(24):12080-12087. doi:10.1021/acs.analchem.5b02801 .
Habtamu, Henok B., Sentić, Milica, Silvestrini, Morena, De Leo, Luigina, Not, Tarcisio, Arbault, Stephane, Manojlović, Dragan D., Šojić, Nešo, Ugo, Paolo, "A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles" in Analytical Chemistry, 87, no. 24 (2015):12080-12087, https://doi.org/10.1021/acs.analchem.5b02801 . .