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dc.creatorPetric, Marija
dc.creatorSubotic, Angelina
dc.creatorJevremovic, Sladana
dc.creatorTrifunović-Momcilov, Milana
dc.creatorTadić, Vojin
dc.creatorGrujić, Marica
dc.creatorVujčić, Zoran
dc.date.accessioned2018-11-22T00:33:28Z
dc.date.available2018-11-22T00:33:28Z
dc.date.issued2015
dc.identifier.issn1631-0691
dc.identifier.urihttps://cherry.chem.bg.ac.rs/handle/123456789/2032
dc.description.abstractMorphogenesis in vitro is a complex and still poorly defined process. We investigated esterase and peroxidase isoforms detected in bulb scale, during Fritillaria meleagris morphogenesis. Bulbs were grown either at 4 degrees C or on a medium with an increased concentration of sucrose (4.5%) for 30 days. After these pre-treatments, the bulb scales were further grown on nutrient media that contained different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) or thidiazuron (TDZ). Regeneration of somatic embryos and bulblets occurred at the same explant. The highest numbers of somatic embryos and bulblets were regenerated on the medium containing 2,4-D and KIN (1 mg/L each), while morphogenesis was most successful at a TDZ concentration between 0.5 and 1 mg/L. Monitoring of esterases and peroxidases was performed by growing bulb scales on a medium enriched with 2,4-D and KIN or TDZ (1 mg/L), and the number and activity of isoforms were followed every 7 days for 4 weeks. In control explants, six isoforms of esterase were observed. Three isoforms of peroxidase were not detected in the control bulb scale, which has not begun its morphogenesis process. 2015 Academie des sciences. (C) Published by Elsevier Masson SAS. All rights reserved.en
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173015/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.rightsrestrictedAccess
dc.sourceComptes Rendus Biologies
dc.subjectSnake's headen
dc.subjectBulbsen
dc.subjectEnzyme activityen
dc.titleEsterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale cultureen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПетриц, Марија; Тадиц, Војин; Грујиц, Марица; Трифуновиц-Момцилов, Милана; Вујчић, Зоран; Суботиц, Aнгелина; Јевремовиц, Сладана;
dc.citation.volume338
dc.citation.issue12
dc.citation.spage793
dc.citation.epage802
dc.identifier.wos000368211700003
dc.identifier.doi10.1016/j.crvi.2015.08.002
dc.citation.other338(12): 793-802
dc.citation.rankM23
dc.identifier.pmid26545851
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84951796790
dc.identifier.rcubKon_2987


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