Anti-cancer Herbal Drug Berberine - Sensitive Determination Using Boron-doped Diamond Electrode
Abstract
Determination of berberine, an isoquinoline plant alkaloid, with antibacterial, antiparasitic, antifungal, hypotensive and antitumoral effects, was proposed by introducing square wave voltammetry on boron-doped diamond electrode. At optimized experimental parameters, in Britton-Robinson buffer solution pH 5 berberine provides 3 oxidation peaks (+0.63; +1.14 and +1.34 V) and one reduction (+0.15 V) ( vs. Ag/AgCl electrode), with good repeatability (relative standard deviation of 2.6% and 1.9% for 8 measurements at 0.5 and 10 mu M concentration level, respectively). Calibration curve was linear in wade linear range from 0.1 to 50 mu M with limit of detection of 0.04 mu M. The proposed procedure was successfully applied for the determination of berberine in seed extract from Argemone mexicana with satisfactory recovery ( 102-102.6%). The developed method may represent a sensitive alternative to highly toxic mercury electrodes, modified electrodes and chromatographic methods.
Keywords:
berberine / boron-doped diamond electrode / Argemone mexicana / square wave voltammetrySource:
Electroanalysis, 2015, 27, 12, 2753-2759Publisher:
- Wiley-V C H Verlag Gmbh, Weinheim
Funding / projects:
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
DOI: 10.1002/elan.201500356
ISSN: 1040-0397
WoS: 000368340300008
Scopus: 2-s2.0-84954316177
Collections
Institution/Community
Inovacioni centar / Innovation CentreTY - JOUR AU - Stanković, Dalibor AU - Samphao, Anchalee AU - Kalcher, Kurt PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2033 AB - Determination of berberine, an isoquinoline plant alkaloid, with antibacterial, antiparasitic, antifungal, hypotensive and antitumoral effects, was proposed by introducing square wave voltammetry on boron-doped diamond electrode. At optimized experimental parameters, in Britton-Robinson buffer solution pH 5 berberine provides 3 oxidation peaks (+0.63; +1.14 and +1.34 V) and one reduction (+0.15 V) ( vs. Ag/AgCl electrode), with good repeatability (relative standard deviation of 2.6% and 1.9% for 8 measurements at 0.5 and 10 mu M concentration level, respectively). Calibration curve was linear in wade linear range from 0.1 to 50 mu M with limit of detection of 0.04 mu M. The proposed procedure was successfully applied for the determination of berberine in seed extract from Argemone mexicana with satisfactory recovery ( 102-102.6%). The developed method may represent a sensitive alternative to highly toxic mercury electrodes, modified electrodes and chromatographic methods. PB - Wiley-V C H Verlag Gmbh, Weinheim T2 - Electroanalysis T1 - Anti-cancer Herbal Drug Berberine - Sensitive Determination Using Boron-doped Diamond Electrode VL - 27 IS - 12 SP - 2753 EP - 2759 DO - 10.1002/elan.201500356 ER -
@article{ author = "Stanković, Dalibor and Samphao, Anchalee and Kalcher, Kurt", year = "2015", abstract = "Determination of berberine, an isoquinoline plant alkaloid, with antibacterial, antiparasitic, antifungal, hypotensive and antitumoral effects, was proposed by introducing square wave voltammetry on boron-doped diamond electrode. At optimized experimental parameters, in Britton-Robinson buffer solution pH 5 berberine provides 3 oxidation peaks (+0.63; +1.14 and +1.34 V) and one reduction (+0.15 V) ( vs. Ag/AgCl electrode), with good repeatability (relative standard deviation of 2.6% and 1.9% for 8 measurements at 0.5 and 10 mu M concentration level, respectively). Calibration curve was linear in wade linear range from 0.1 to 50 mu M with limit of detection of 0.04 mu M. The proposed procedure was successfully applied for the determination of berberine in seed extract from Argemone mexicana with satisfactory recovery ( 102-102.6%). The developed method may represent a sensitive alternative to highly toxic mercury electrodes, modified electrodes and chromatographic methods.", publisher = "Wiley-V C H Verlag Gmbh, Weinheim", journal = "Electroanalysis", title = "Anti-cancer Herbal Drug Berberine - Sensitive Determination Using Boron-doped Diamond Electrode", volume = "27", number = "12", pages = "2753-2759", doi = "10.1002/elan.201500356" }
Stanković, D., Samphao, A.,& Kalcher, K.. (2015). Anti-cancer Herbal Drug Berberine - Sensitive Determination Using Boron-doped Diamond Electrode. in Electroanalysis Wiley-V C H Verlag Gmbh, Weinheim., 27(12), 2753-2759. https://doi.org/10.1002/elan.201500356
Stanković D, Samphao A, Kalcher K. Anti-cancer Herbal Drug Berberine - Sensitive Determination Using Boron-doped Diamond Electrode. in Electroanalysis. 2015;27(12):2753-2759. doi:10.1002/elan.201500356 .
Stanković, Dalibor, Samphao, Anchalee, Kalcher, Kurt, "Anti-cancer Herbal Drug Berberine - Sensitive Determination Using Boron-doped Diamond Electrode" in Electroanalysis, 27, no. 12 (2015):2753-2759, https://doi.org/10.1002/elan.201500356 . .