Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase
Samo za registrovane korisnike
2016
Autori
Mitić-Ćulafić, DraganaNikolić, Biljana
Simin, Nataša
Jasnić, Nebojša
Četojević-Simin, Dragana
Krstić-Ristivojević, Maja
Knežević-Vukčević, Jelena
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Allium flavum L. and Allium melanantherum Pan. are wild growing plants used in traditional diet in Balkan region. While chemical composition and some biological activities of A. flavum have been reported, A. melanantherum, as an endemic in the Balkan Peninsula, has never been comprehensively examined. After chemical characterization of A. melanantherum, we examined the protective effect of methanol extracts of both species against t-butyl hydro-peroxide (t-BOOH)-induced DNA damage and mutagenesis. The bacterial reverse mutation assay was performed on Escherichia coli WP2 oxyR strain. DNA damage was monitored in human fetal lung fibroblasts (MRC-5) with alkaline comet assay. Obtained results indicated that extracts reduced t-BOOH-induced DNA damage up to 70 and 72 % for A. flavum and A. melanantherum extract, respectively, and showed no effect on t-BOOH-induced mutagenesis. Since the results indicated modulatory effect on cell-mediated antioxidative defense, the effect of extracts on to...tal protein content, and superoxide dismutase (SOD) and catalase (CAT) amounts and activities were monitored. Both extracts increased total protein content, while the increase of enzyme amount and activity was obtained only with A. melanantherum extract and restricted to CAT. The activity of CuZnSOD family was not affected, while SOD1 and SOD2 amounts were significantly decreased, indicating potential involvement of extracellular CuZnSOD. Obtained results strongly support the traditional use of A. flavum and A. melanantherum in nutrition and recommend them for further study.
Ključne reči:
Allium flavum / Allium melanantherum / Antigenotoxicity / Oxidative DNA damage / Antioxidative enzymesIzvor:
Plant Foods for Human Nutrition, 2016, 71, 1, 28-34Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- Biološki aktivni prirodni proizvodi kao potencijalni izvori novih lekova i dijetetskih suplemenata (RS-MESTD-Basic Research (BR or ON)-172058)
Napomena:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3482
DOI: 10.1007/s11130-015-0519-0
ISSN: 0921-9668
PubMed: 26590605
WoS: 000372290500004
Scopus: 2-s2.0-84961123592
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Mitić-Ćulafić, Dragana AU - Nikolić, Biljana AU - Simin, Nataša AU - Jasnić, Nebojša AU - Četojević-Simin, Dragana AU - Krstić-Ristivojević, Maja AU - Knežević-Vukčević, Jelena PY - 2016 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2065 AB - Allium flavum L. and Allium melanantherum Pan. are wild growing plants used in traditional diet in Balkan region. While chemical composition and some biological activities of A. flavum have been reported, A. melanantherum, as an endemic in the Balkan Peninsula, has never been comprehensively examined. After chemical characterization of A. melanantherum, we examined the protective effect of methanol extracts of both species against t-butyl hydro-peroxide (t-BOOH)-induced DNA damage and mutagenesis. The bacterial reverse mutation assay was performed on Escherichia coli WP2 oxyR strain. DNA damage was monitored in human fetal lung fibroblasts (MRC-5) with alkaline comet assay. Obtained results indicated that extracts reduced t-BOOH-induced DNA damage up to 70 and 72 % for A. flavum and A. melanantherum extract, respectively, and showed no effect on t-BOOH-induced mutagenesis. Since the results indicated modulatory effect on cell-mediated antioxidative defense, the effect of extracts on total protein content, and superoxide dismutase (SOD) and catalase (CAT) amounts and activities were monitored. Both extracts increased total protein content, while the increase of enzyme amount and activity was obtained only with A. melanantherum extract and restricted to CAT. The activity of CuZnSOD family was not affected, while SOD1 and SOD2 amounts were significantly decreased, indicating potential involvement of extracellular CuZnSOD. Obtained results strongly support the traditional use of A. flavum and A. melanantherum in nutrition and recommend them for further study. PB - Springer, Dordrecht T2 - Plant Foods for Human Nutrition T1 - Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase VL - 71 IS - 1 SP - 28 EP - 34 DO - 10.1007/s11130-015-0519-0 ER -
@article{ author = "Mitić-Ćulafić, Dragana and Nikolić, Biljana and Simin, Nataša and Jasnić, Nebojša and Četojević-Simin, Dragana and Krstić-Ristivojević, Maja and Knežević-Vukčević, Jelena", year = "2016", abstract = "Allium flavum L. and Allium melanantherum Pan. are wild growing plants used in traditional diet in Balkan region. While chemical composition and some biological activities of A. flavum have been reported, A. melanantherum, as an endemic in the Balkan Peninsula, has never been comprehensively examined. After chemical characterization of A. melanantherum, we examined the protective effect of methanol extracts of both species against t-butyl hydro-peroxide (t-BOOH)-induced DNA damage and mutagenesis. The bacterial reverse mutation assay was performed on Escherichia coli WP2 oxyR strain. DNA damage was monitored in human fetal lung fibroblasts (MRC-5) with alkaline comet assay. Obtained results indicated that extracts reduced t-BOOH-induced DNA damage up to 70 and 72 % for A. flavum and A. melanantherum extract, respectively, and showed no effect on t-BOOH-induced mutagenesis. Since the results indicated modulatory effect on cell-mediated antioxidative defense, the effect of extracts on total protein content, and superoxide dismutase (SOD) and catalase (CAT) amounts and activities were monitored. Both extracts increased total protein content, while the increase of enzyme amount and activity was obtained only with A. melanantherum extract and restricted to CAT. The activity of CuZnSOD family was not affected, while SOD1 and SOD2 amounts were significantly decreased, indicating potential involvement of extracellular CuZnSOD. Obtained results strongly support the traditional use of A. flavum and A. melanantherum in nutrition and recommend them for further study.", publisher = "Springer, Dordrecht", journal = "Plant Foods for Human Nutrition", title = "Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase", volume = "71", number = "1", pages = "28-34", doi = "10.1007/s11130-015-0519-0" }
Mitić-Ćulafić, D., Nikolić, B., Simin, N., Jasnić, N., Četojević-Simin, D., Krstić-Ristivojević, M.,& Knežević-Vukčević, J.. (2016). Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. in Plant Foods for Human Nutrition Springer, Dordrecht., 71(1), 28-34. https://doi.org/10.1007/s11130-015-0519-0
Mitić-Ćulafić D, Nikolić B, Simin N, Jasnić N, Četojević-Simin D, Krstić-Ristivojević M, Knežević-Vukčević J. Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. in Plant Foods for Human Nutrition. 2016;71(1):28-34. doi:10.1007/s11130-015-0519-0 .
Mitić-Ćulafić, Dragana, Nikolić, Biljana, Simin, Nataša, Jasnić, Nebojša, Četojević-Simin, Dragana, Krstić-Ristivojević, Maja, Knežević-Vukčević, Jelena, "Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase" in Plant Foods for Human Nutrition, 71, no. 1 (2016):28-34, https://doi.org/10.1007/s11130-015-0519-0 . .