Immobilization of glucoamylase on macroporous spheres
Imobilizacija glukoamilaze na makroporoznim sferama
Апстракт
Glucoamylase was covalently immobilized through the spacer-arm of the poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) spheres by using a glutaraldehyde as a coupling agent. The influence of the enzyme load applied to the support on immobilization, yield and specific activity, has been determined. Obtained specific activity was 700 U/g with immobilization yield of 35 %. The Km value for immobilized glucoamylase was 1.28 % (w/v), pH and temperature optimum were 4.5 and 70°C, respectively. The conversion of 20 % (w/w) starch hydrolysate achieved with the immobilized glucoamylase was 97 % after 5 hours.
Glukoamilaza je imobilizovana preko spejsera na sfere kopolimera glicidil metakrilata i etilen glikol dimetakrilata uz pomoć glutaraldehida. Određen je uticaj količine dodatog enzima na prinos imobilizacije kao i na specifičnu aktivnost dobijenog imobilizata. Dobijena je specifična aktivnost od 700 U/g sa prinosom imobilizacije od 35%. Km vrednost imobilizovane glukoamilaze je 1,28 % (w/v), pH i temperaturni optimumi su 4,5 i 70°S. Imobilizovani enzim je pri hidrolizi 20 % (w/w) hidrolizata skroba postigao konverziju od 97 % nakon 5 sati.
Кључне речи:
Glucoamylase / immobilization / poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)Извор:
Acta periodica technologica, 2004, 35, 207-214Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Milosavić, Nenad B. AU - Prodanović, Radivoje AU - Jovanović, Slobodan M. AU - Vujčić, Zoran PY - 2004 UR - https://cherry.chem.bg.ac.rs/handle/123456789/209 AB - Glucoamylase was covalently immobilized through the spacer-arm of the poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) spheres by using a glutaraldehyde as a coupling agent. The influence of the enzyme load applied to the support on immobilization, yield and specific activity, has been determined. Obtained specific activity was 700 U/g with immobilization yield of 35 %. The Km value for immobilized glucoamylase was 1.28 % (w/v), pH and temperature optimum were 4.5 and 70°C, respectively. The conversion of 20 % (w/w) starch hydrolysate achieved with the immobilized glucoamylase was 97 % after 5 hours. AB - Glukoamilaza je imobilizovana preko spejsera na sfere kopolimera glicidil metakrilata i etilen glikol dimetakrilata uz pomoć glutaraldehida. Određen je uticaj količine dodatog enzima na prinos imobilizacije kao i na specifičnu aktivnost dobijenog imobilizata. Dobijena je specifična aktivnost od 700 U/g sa prinosom imobilizacije od 35%. Km vrednost imobilizovane glukoamilaze je 1,28 % (w/v), pH i temperaturni optimumi su 4,5 i 70°S. Imobilizovani enzim je pri hidrolizi 20 % (w/w) hidrolizata skroba postigao konverziju od 97 % nakon 5 sati. T2 - Acta periodica technologica T1 - Immobilization of glucoamylase on macroporous spheres T1 - Imobilizacija glukoamilaze na makroporoznim sferama IS - 35 SP - 207 EP - 214 DO - 10.2298/APT0435207M ER -
@article{ author = "Milosavić, Nenad B. and Prodanović, Radivoje and Jovanović, Slobodan M. and Vujčić, Zoran", year = "2004", abstract = "Glucoamylase was covalently immobilized through the spacer-arm of the poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) spheres by using a glutaraldehyde as a coupling agent. The influence of the enzyme load applied to the support on immobilization, yield and specific activity, has been determined. Obtained specific activity was 700 U/g with immobilization yield of 35 %. The Km value for immobilized glucoamylase was 1.28 % (w/v), pH and temperature optimum were 4.5 and 70°C, respectively. The conversion of 20 % (w/w) starch hydrolysate achieved with the immobilized glucoamylase was 97 % after 5 hours., Glukoamilaza je imobilizovana preko spejsera na sfere kopolimera glicidil metakrilata i etilen glikol dimetakrilata uz pomoć glutaraldehida. Određen je uticaj količine dodatog enzima na prinos imobilizacije kao i na specifičnu aktivnost dobijenog imobilizata. Dobijena je specifična aktivnost od 700 U/g sa prinosom imobilizacije od 35%. Km vrednost imobilizovane glukoamilaze je 1,28 % (w/v), pH i temperaturni optimumi su 4,5 i 70°S. Imobilizovani enzim je pri hidrolizi 20 % (w/w) hidrolizata skroba postigao konverziju od 97 % nakon 5 sati.", journal = "Acta periodica technologica", title = "Immobilization of glucoamylase on macroporous spheres, Imobilizacija glukoamilaze na makroporoznim sferama", number = "35", pages = "207-214", doi = "10.2298/APT0435207M" }
Milosavić, N. B., Prodanović, R., Jovanović, S. M.,& Vujčić, Z.. (2004). Immobilization of glucoamylase on macroporous spheres. in Acta periodica technologica(35), 207-214. https://doi.org/10.2298/APT0435207M
Milosavić NB, Prodanović R, Jovanović SM, Vujčić Z. Immobilization of glucoamylase on macroporous spheres. in Acta periodica technologica. 2004;(35):207-214. doi:10.2298/APT0435207M .
Milosavić, Nenad B., Prodanović, Radivoje, Jovanović, Slobodan M., Vujčić, Zoran, "Immobilization of glucoamylase on macroporous spheres" in Acta periodica technologica, no. 35 (2004):207-214, https://doi.org/10.2298/APT0435207M . .