Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana
Само за регистроване кориснике
2018
Аутори
Nikolić, JasnaNešić, Andrijana N.
Kull, Skadi
Schocker, Frauke
Jappe, Uta
Gavrović-Jankulović, Marija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminate) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase. A recombinant homologue of natural catalase was produced, isolated and biochemically characterized. The recombinant protein showed IgE reactivity in 7 out of 13 tested patients with suspected allergy to banana in immunoblot. Novel banana fruit allergens should be added as components to allergen-microarrays for the diagnosis and the ...monitoring of banana allergy. Significance: By employment of CPLL methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis catalase from banana fruit is identified as a novel allergen, with proposed designation as Mus a 7. IgE reactive recombinant Mus a 7 was produced and should be included in a component-resolved allergy diagnosis.
Кључне речи:
Allergens / Banana allergy / Catalase / Combinatorial peptide ligand library / 2-D PAGEИзвор:
Journal of Proteomics, 2018, 175, 87-94Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-172049)
- Bioanalytical Chemistry Research Group at the Research Center Borstel
- German Academic Exchange Service DAAD Research [91530210-50015537]
Напомена:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3203
DOI: 10.1016/j.jprot.2018.01.007
ISSN: 1874-3919
PubMed: 29331514
WoS: 000428489600010
Scopus: 2-s2.0-85040595552
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Nikolić, Jasna AU - Nešić, Andrijana N. AU - Kull, Skadi AU - Schocker, Frauke AU - Jappe, Uta AU - Gavrović-Jankulović, Marija PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2117 AB - Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminate) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase. A recombinant homologue of natural catalase was produced, isolated and biochemically characterized. The recombinant protein showed IgE reactivity in 7 out of 13 tested patients with suspected allergy to banana in immunoblot. Novel banana fruit allergens should be added as components to allergen-microarrays for the diagnosis and the monitoring of banana allergy. Significance: By employment of CPLL methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis catalase from banana fruit is identified as a novel allergen, with proposed designation as Mus a 7. IgE reactive recombinant Mus a 7 was produced and should be included in a component-resolved allergy diagnosis. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Proteomics T1 - Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana VL - 175 SP - 87 EP - 94 DO - 10.1016/j.jprot.2018.01.007 ER -
@article{ author = "Nikolić, Jasna and Nešić, Andrijana N. and Kull, Skadi and Schocker, Frauke and Jappe, Uta and Gavrović-Jankulović, Marija", year = "2018", abstract = "Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminate) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase. A recombinant homologue of natural catalase was produced, isolated and biochemically characterized. The recombinant protein showed IgE reactivity in 7 out of 13 tested patients with suspected allergy to banana in immunoblot. Novel banana fruit allergens should be added as components to allergen-microarrays for the diagnosis and the monitoring of banana allergy. Significance: By employment of CPLL methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis catalase from banana fruit is identified as a novel allergen, with proposed designation as Mus a 7. IgE reactive recombinant Mus a 7 was produced and should be included in a component-resolved allergy diagnosis.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Proteomics", title = "Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana", volume = "175", pages = "87-94", doi = "10.1016/j.jprot.2018.01.007" }
Nikolić, J., Nešić, A. N., Kull, S., Schocker, F., Jappe, U.,& Gavrović-Jankulović, M.. (2018). Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana. in Journal of Proteomics Elsevier Science Bv, Amsterdam., 175, 87-94. https://doi.org/10.1016/j.jprot.2018.01.007
Nikolić J, Nešić AN, Kull S, Schocker F, Jappe U, Gavrović-Jankulović M. Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana. in Journal of Proteomics. 2018;175:87-94. doi:10.1016/j.jprot.2018.01.007 .
Nikolić, Jasna, Nešić, Andrijana N., Kull, Skadi, Schocker, Frauke, Jappe, Uta, Gavrović-Jankulović, Marija, "Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana" in Journal of Proteomics, 175 (2018):87-94, https://doi.org/10.1016/j.jprot.2018.01.007 . .