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dc.creatorIzrael-Živković, Lidija
dc.creatorRikalović, Milena G.
dc.creatorGojgić-Cvijović, Gordana D.
dc.creatorKazazić, Saša
dc.creatorVrvić, Miroslav M.
dc.creatorBrčeski, Ilija
dc.creatorBeškoski, Vladimir
dc.creatorLončarević, Branka D.
dc.creatorGopčević, Kristina
dc.creatorKaradžić, Ivanka M.
dc.date.accessioned2018-11-22T00:43:45Z
dc.date.available2018-11-22T00:43:45Z
dc.date.issued2018
dc.identifier.issn2046-2069
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/2118
dc.description.abstractPseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture versus control was found. Our results signify that P. aeruginosa san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.en
dc.publisherRoyal Soc Chemistry, Cambridge
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS//
dc.rightsopenAccess
dc.sourceRSC Advances
dc.titleCadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san aien
dc.typearticle
dc.rights.licenseBY
dcterms.abstractИзраел-Зивковиц, Лидија; Бешкоски, Владимир; Брчески, Илија; Гопцевиц, Кристина; Лонцаревиц, Бранка; Казазиц, Саса; Рикаловиц, Милена; Гојгиц-Цвијовиц, Гордана; Врвиц, Мирослав; Карадзиц, Иванка;
dc.citation.volume8
dc.citation.issue19
dc.citation.spage10549
dc.citation.epage10560
dc.identifier.wos000428581000023
dc.identifier.doi10.1039/c8ra00371h
dc.citation.other8(19): 10549-10560
dc.citation.rankM22
dc.description.otherSupplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3179]
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85044180168
dc.identifier.fulltexthttp://cherry.chem.bg.ac.rs/bitstream/id/9137/2116.pdf
dc.identifier.rcubKon_3449


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