Comparative solution equilibrium and structural studies of half-sandwich ruthenium(II)(eta(6)-toluene) complexes of picolinate derivatives
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Gal, Tamas G.
May, Nora V.
Enyedy, Eva A.
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Five Ru(II)(eta(6)-toluene) complexes formed with 2-picolinic acid and its various derivatives have been synthesized and characterized. X-ray structures of four complexes are also reported. Complex formation processes of [Ru(II) (eta(6)-toluene)(H2O)(3)](2+) organometallic cation with the metal-free ligands were studied in aqueous solution in the presence of chloride ions by the combined use of H-1 NMR spectroscopy, UV-visible spectrophotometry and pH-potentiometry. Solution stability, chloride ion affinity and lipophilicity of the complexes were characterized together with in vitro cytotoxic and antiproliferative activity in cancer cell lines being sensitive and resistant to classic chemotherapy and in normal cells as well. Formation of mono complexes such as [Ru(eta(6)-toluene)(L) (Z)](+/0) (L: completely deprotonated ligand; Z = H2O/Cl-) with high stability and [Ru(eta(6)-toluene)(L)(OH)] was found in solution. The plc values (8.3-8.7) reflect the formation of low amount of mixed hy...droxido species at pH 7.4 at 0.2 M KCl ionic strength. The complexes are fairly hydrophilic and show moderate chloride ion affinity and fast chloride-water exchange processes. The studied complexes exhibit no cytotoxic activity in human cancer cells (IC50 gt 100 mu M), only complexes formed with 2-picolinic acid (1) and its 3-methyl derivative (2) represented a moderate antiproliferative effect (IC50 = 84.8 (1), 79.2 mu M (2)) on a multidrug resistant colon adenocarcinoma cell line revealing considerable multidrug resistant selectivity. Complexes 1 and 2 bind to human serum albumin covalently and relatively slowly with moderate strength at multiple binding sites without ligand cleavage.
Ključne reči:Stability constants / X-ray crystal structures / Half-sandwich complexes / Speciation / Antiproliferative activity
Izvor:Journal of Inorganic Biochemistry, 2018, 181, 74-85
- Elsevier Science Inc, New York
- Racionalni dizajn i sinteza biološki aktivnih i koordinacionih jedinjenja i funkcionalnih materijala, relevantnih u (bio)nanotehnologiji (RS-172035)
- Hungarian Academy of Sciences
- Ministry of Human Capacities [UNKP-17-4]
- National Research, Development and Innovation Office-NKFIA [GINOP-2.3.2-15-2016-00038, FK 124240, K 115762]
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3123