In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes
Само за регистроване кориснике
2018
Аутори
Jurica, KarloBrčić-Karačonji, Irena
Mikolić, Anja
Milojković-Opsenica, Dušanka
Benković, Vesna
Kopjar, Nevenka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Strawberry tree (Arbutus unedo L.) leaves have long been used in the traditional medicine of the Mediterranean region. One of their most bioactive constituents is the glycoside arbutin, whose presence makes A. unedo suitable as a potential substitute for bearberry [Arctostaphylos uva ursi (L.) Spreng] leaves, an herbal preparation widely used for treating urinary tract infections. The safety and biocompatibility of strawberry tree water leaf extract have not yet been documented well. This study estimated arbutin content in strawberry tree water leaf extract (STE) using high performance liquid chromatography. Furthermore, we performed an in vitro safety assessment of the 24 h exposure to three presumably non-toxic concentrations of standardized STE and arbutin in human peripheral blood lymphocytes using the apoptosis/necrosis assay, the alkaline comet assay, and the cytokinesis-block micronucleus cytome assay. The STE was also tested for total antioxidant capacity and lipid peroxidation.... At a concentration corresponding to the maximum allowable daily intake of arbutin, the tested extract was not cytotoxic, had a negligible potential for causing primary DNA damage and even hindered micronuclei formation in lymphocytes. It also showed a valuable antioxidant capacity, and did not exert marked lipid peroxidation. These promising results represent a solid frame for further development of STE-based herbal preparations. Although arbutin generally had a low DNA damaging potential, the slowing down of lymphocyte proliferation observed after 24 h of exposure points to a cytostatic effect, which merits further research.
Кључне речи:
Apoptosis / Arbutus unedo L. / Cytokinesis-block micronucleus cytome assay / Oxidative stress / Primary DNA damageИзвор:
Cytotechnology, 2018, 70, 4, 1261-1278Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Ministry of Science and Education of the Republic of Croatia
- Mali Losinj Tourist Board
DOI: 10.1007/s10616-018-0218-4
ISSN: 0920-9069
PubMed: 29696482
WoS: 000440814500014
Scopus: 2-s2.0-85046026118
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Jurica, Karlo AU - Brčić-Karačonji, Irena AU - Mikolić, Anja AU - Milojković-Opsenica, Dušanka AU - Benković, Vesna AU - Kopjar, Nevenka PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2194 AB - Strawberry tree (Arbutus unedo L.) leaves have long been used in the traditional medicine of the Mediterranean region. One of their most bioactive constituents is the glycoside arbutin, whose presence makes A. unedo suitable as a potential substitute for bearberry [Arctostaphylos uva ursi (L.) Spreng] leaves, an herbal preparation widely used for treating urinary tract infections. The safety and biocompatibility of strawberry tree water leaf extract have not yet been documented well. This study estimated arbutin content in strawberry tree water leaf extract (STE) using high performance liquid chromatography. Furthermore, we performed an in vitro safety assessment of the 24 h exposure to three presumably non-toxic concentrations of standardized STE and arbutin in human peripheral blood lymphocytes using the apoptosis/necrosis assay, the alkaline comet assay, and the cytokinesis-block micronucleus cytome assay. The STE was also tested for total antioxidant capacity and lipid peroxidation. At a concentration corresponding to the maximum allowable daily intake of arbutin, the tested extract was not cytotoxic, had a negligible potential for causing primary DNA damage and even hindered micronuclei formation in lymphocytes. It also showed a valuable antioxidant capacity, and did not exert marked lipid peroxidation. These promising results represent a solid frame for further development of STE-based herbal preparations. Although arbutin generally had a low DNA damaging potential, the slowing down of lymphocyte proliferation observed after 24 h of exposure points to a cytostatic effect, which merits further research. PB - Springer, Dordrecht T2 - Cytotechnology T1 - In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes VL - 70 IS - 4 SP - 1261 EP - 1278 DO - 10.1007/s10616-018-0218-4 ER -
@article{ author = "Jurica, Karlo and Brčić-Karačonji, Irena and Mikolić, Anja and Milojković-Opsenica, Dušanka and Benković, Vesna and Kopjar, Nevenka", year = "2018", abstract = "Strawberry tree (Arbutus unedo L.) leaves have long been used in the traditional medicine of the Mediterranean region. One of their most bioactive constituents is the glycoside arbutin, whose presence makes A. unedo suitable as a potential substitute for bearberry [Arctostaphylos uva ursi (L.) Spreng] leaves, an herbal preparation widely used for treating urinary tract infections. The safety and biocompatibility of strawberry tree water leaf extract have not yet been documented well. This study estimated arbutin content in strawberry tree water leaf extract (STE) using high performance liquid chromatography. Furthermore, we performed an in vitro safety assessment of the 24 h exposure to three presumably non-toxic concentrations of standardized STE and arbutin in human peripheral blood lymphocytes using the apoptosis/necrosis assay, the alkaline comet assay, and the cytokinesis-block micronucleus cytome assay. The STE was also tested for total antioxidant capacity and lipid peroxidation. At a concentration corresponding to the maximum allowable daily intake of arbutin, the tested extract was not cytotoxic, had a negligible potential for causing primary DNA damage and even hindered micronuclei formation in lymphocytes. It also showed a valuable antioxidant capacity, and did not exert marked lipid peroxidation. These promising results represent a solid frame for further development of STE-based herbal preparations. Although arbutin generally had a low DNA damaging potential, the slowing down of lymphocyte proliferation observed after 24 h of exposure points to a cytostatic effect, which merits further research.", publisher = "Springer, Dordrecht", journal = "Cytotechnology", title = "In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes", volume = "70", number = "4", pages = "1261-1278", doi = "10.1007/s10616-018-0218-4" }
Jurica, K., Brčić-Karačonji, I., Mikolić, A., Milojković-Opsenica, D., Benković, V.,& Kopjar, N.. (2018). In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes. in Cytotechnology Springer, Dordrecht., 70(4), 1261-1278. https://doi.org/10.1007/s10616-018-0218-4
Jurica K, Brčić-Karačonji I, Mikolić A, Milojković-Opsenica D, Benković V, Kopjar N. In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes. in Cytotechnology. 2018;70(4):1261-1278. doi:10.1007/s10616-018-0218-4 .
Jurica, Karlo, Brčić-Karačonji, Irena, Mikolić, Anja, Milojković-Opsenica, Dušanka, Benković, Vesna, Kopjar, Nevenka, "In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes" in Cytotechnology, 70, no. 4 (2018):1261-1278, https://doi.org/10.1007/s10616-018-0218-4 . .