RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing
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2018
Authors
Vukojević, VesnaĐurđić, Slađana Z.
Ognjanović, Miloš
Antić, Bratislav
Kalcher, Kurt
Mutić, Jelena
Stanković, Dalibor
Article (Published version)
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In this work, we aimed to propose a newly synthesized composite material with enhanced electrocatalytic properties as a novel screen-printed sensor for the quantification of NADH. Additionally, the surface was modified with alcohol dehydrogenase for the preparation of an amperometric biosensor for analysis of ethanol. Synthesized material was characterized using several microstructural (FE-SEM, HR-TEM, XRD) and electrochemical (CV, EIS) techniques. The electrochemical response of the tested analytes was investigated as a function of important parameters. Under optimal conditions, the working linear range and limit of detection for ethanol sensing was 1-1800 mu M and 0.19 mu M, respectively. For NADH, the linear range was from 1 to 1300 mu M with limit of detection of 0.52 mu M. Moreover, effects of some possible interfering compounds were investigated and the developed procedure was applied to commercial alcoholic beverages. The results obtained showed satisfactory precision and accura...cy of the developed method and confirm the proposed approach could be a possible replacement for the currently used techniques for ethanol and NADH quantification.
Keywords:
Ethanol biosensor / Screen printed electrode / Graphene nanoribbons / Alcohol dehydrogenaseSource:
Biosensors and Bioelectronics, 2018, 117, 392-397Publisher:
- Elsevier Advanced Technology, Oxford
Funding / projects:
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-FP7-621375)
- Education of Modern Analytical and Bioanalytical Methods
- CEEPUS [CIII-CZ-0212-11-1718]
Note:
- Peer-reviewed manuscript: http://cherry.chem.bg.ac.rs/handle/123456789/2985
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/2986
DOI: 10.1016/j.bios.2018.06.038
ISSN: 0956-5663
PubMed: 29960271
WoS: 000442191900047
Scopus: 2-s2.0-85049075205
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Vukojević, Vesna AU - Đurđić, Slađana Z. AU - Ognjanović, Miloš AU - Antić, Bratislav AU - Kalcher, Kurt AU - Mutić, Jelena AU - Stanković, Dalibor PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2204 AB - In this work, we aimed to propose a newly synthesized composite material with enhanced electrocatalytic properties as a novel screen-printed sensor for the quantification of NADH. Additionally, the surface was modified with alcohol dehydrogenase for the preparation of an amperometric biosensor for analysis of ethanol. Synthesized material was characterized using several microstructural (FE-SEM, HR-TEM, XRD) and electrochemical (CV, EIS) techniques. The electrochemical response of the tested analytes was investigated as a function of important parameters. Under optimal conditions, the working linear range and limit of detection for ethanol sensing was 1-1800 mu M and 0.19 mu M, respectively. For NADH, the linear range was from 1 to 1300 mu M with limit of detection of 0.52 mu M. Moreover, effects of some possible interfering compounds were investigated and the developed procedure was applied to commercial alcoholic beverages. The results obtained showed satisfactory precision and accuracy of the developed method and confirm the proposed approach could be a possible replacement for the currently used techniques for ethanol and NADH quantification. PB - Elsevier Advanced Technology, Oxford T2 - Biosensors and Bioelectronics T1 - RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing VL - 117 SP - 392 EP - 397 DO - 10.1016/j.bios.2018.06.038 ER -
@article{ author = "Vukojević, Vesna and Đurđić, Slađana Z. and Ognjanović, Miloš and Antić, Bratislav and Kalcher, Kurt and Mutić, Jelena and Stanković, Dalibor", year = "2018", abstract = "In this work, we aimed to propose a newly synthesized composite material with enhanced electrocatalytic properties as a novel screen-printed sensor for the quantification of NADH. Additionally, the surface was modified with alcohol dehydrogenase for the preparation of an amperometric biosensor for analysis of ethanol. Synthesized material was characterized using several microstructural (FE-SEM, HR-TEM, XRD) and electrochemical (CV, EIS) techniques. The electrochemical response of the tested analytes was investigated as a function of important parameters. Under optimal conditions, the working linear range and limit of detection for ethanol sensing was 1-1800 mu M and 0.19 mu M, respectively. For NADH, the linear range was from 1 to 1300 mu M with limit of detection of 0.52 mu M. Moreover, effects of some possible interfering compounds were investigated and the developed procedure was applied to commercial alcoholic beverages. The results obtained showed satisfactory precision and accuracy of the developed method and confirm the proposed approach could be a possible replacement for the currently used techniques for ethanol and NADH quantification.", publisher = "Elsevier Advanced Technology, Oxford", journal = "Biosensors and Bioelectronics", title = "RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing", volume = "117", pages = "392-397", doi = "10.1016/j.bios.2018.06.038" }
Vukojević, V., Đurđić, S. Z., Ognjanović, M., Antić, B., Kalcher, K., Mutić, J.,& Stanković, D.. (2018). RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing. in Biosensors and Bioelectronics Elsevier Advanced Technology, Oxford., 117, 392-397. https://doi.org/10.1016/j.bios.2018.06.038
Vukojević V, Đurđić SZ, Ognjanović M, Antić B, Kalcher K, Mutić J, Stanković D. RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing. in Biosensors and Bioelectronics. 2018;117:392-397. doi:10.1016/j.bios.2018.06.038 .
Vukojević, Vesna, Đurđić, Slađana Z., Ognjanović, Miloš, Antić, Bratislav, Kalcher, Kurt, Mutić, Jelena, Stanković, Dalibor, "RuO2/graphene nanoribbon composite supported on screen printed electrode with enhanced electrocatalytic performances toward ethanol and NADH biosensing" in Biosensors and Bioelectronics, 117 (2018):392-397, https://doi.org/10.1016/j.bios.2018.06.038 . .