alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells
2018
Аутори
Krstić-Ristivojević, MajaGrundström, Jeanette
Tran, Thi Anh Thu
Apostolović, Danijela
Radoi, Vlad
Starkhammar, Maria
Vukojević, Vladana
Ćirković-Veličković, Tanja
Hamsten, Carl
van Hage, Marianne
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Red meat allergy is characterized by an IgE response against the carbohydrate galactose-alpha-1,3-galactose (alpha-Gal), which is abundantly expressed on glycoproteins from non-primate mammals. The mechanisms of how alpha-Gal is processed and presented to the immune system to initiate an allergic reaction are still unknown. The aim of this study was to reveal whether the presence of alpha-Gal epitopes on the protein surface influence antigen uptake and processing in immature monocyte-derived dendritic cells (iMDDCs). Immature MDDCs were prepared from healthy blood donors and red meat allergic patients. We found an increased internalization of alpha-Gal carrying proteins over time in iMDDCs by flow cytometric analysis, which was independent of the donor allergic status. The uptake of alpha-Gal carrying proteins was significantly higher than the uptake of non-alpha-Gal carrying proteins. Confocal microscopy revealed alpha-Gal carrying proteins scattered around the cytoplasm in most iMDDC...s while detection of proteins not carrying alpha-Gal was negligible. Fluorescent detection of protein on SDS-PAGE showed that degradation of alpha-Gal carrying proteins was slower than degradation of non-alpha-Gal carrying proteins. Thus, the presence of alpha-Gal on the protein surface affects both uptake and degradation of the protein, and the results add new knowledge of alpha-Gal as a clinically relevant food allergen.
Извор:
Scientific Reports, 2018, 8Издавач:
- Nature Publishing Group, London
Финансирање / пројекти:
- United States Department of Agriculture under Agricultural Research Service [5348-32000-033-00D]
Напомена:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3073
DOI: 10.1038/s41598-018-30887-8
ISSN: 2045-2322
PubMed: 30139949
WoS: 000442530000048
Scopus: 2-s2.0-85052192658
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Krstić-Ristivojević, Maja AU - Grundström, Jeanette AU - Tran, Thi Anh Thu AU - Apostolović, Danijela AU - Radoi, Vlad AU - Starkhammar, Maria AU - Vukojević, Vladana AU - Ćirković-Veličković, Tanja AU - Hamsten, Carl AU - van Hage, Marianne PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2207 AB - Red meat allergy is characterized by an IgE response against the carbohydrate galactose-alpha-1,3-galactose (alpha-Gal), which is abundantly expressed on glycoproteins from non-primate mammals. The mechanisms of how alpha-Gal is processed and presented to the immune system to initiate an allergic reaction are still unknown. The aim of this study was to reveal whether the presence of alpha-Gal epitopes on the protein surface influence antigen uptake and processing in immature monocyte-derived dendritic cells (iMDDCs). Immature MDDCs were prepared from healthy blood donors and red meat allergic patients. We found an increased internalization of alpha-Gal carrying proteins over time in iMDDCs by flow cytometric analysis, which was independent of the donor allergic status. The uptake of alpha-Gal carrying proteins was significantly higher than the uptake of non-alpha-Gal carrying proteins. Confocal microscopy revealed alpha-Gal carrying proteins scattered around the cytoplasm in most iMDDCs while detection of proteins not carrying alpha-Gal was negligible. Fluorescent detection of protein on SDS-PAGE showed that degradation of alpha-Gal carrying proteins was slower than degradation of non-alpha-Gal carrying proteins. Thus, the presence of alpha-Gal on the protein surface affects both uptake and degradation of the protein, and the results add new knowledge of alpha-Gal as a clinically relevant food allergen. PB - Nature Publishing Group, London T2 - Scientific Reports T1 - alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells VL - 8 DO - 10.1038/s41598-018-30887-8 ER -
@article{ author = "Krstić-Ristivojević, Maja and Grundström, Jeanette and Tran, Thi Anh Thu and Apostolović, Danijela and Radoi, Vlad and Starkhammar, Maria and Vukojević, Vladana and Ćirković-Veličković, Tanja and Hamsten, Carl and van Hage, Marianne", year = "2018", abstract = "Red meat allergy is characterized by an IgE response against the carbohydrate galactose-alpha-1,3-galactose (alpha-Gal), which is abundantly expressed on glycoproteins from non-primate mammals. The mechanisms of how alpha-Gal is processed and presented to the immune system to initiate an allergic reaction are still unknown. The aim of this study was to reveal whether the presence of alpha-Gal epitopes on the protein surface influence antigen uptake and processing in immature monocyte-derived dendritic cells (iMDDCs). Immature MDDCs were prepared from healthy blood donors and red meat allergic patients. We found an increased internalization of alpha-Gal carrying proteins over time in iMDDCs by flow cytometric analysis, which was independent of the donor allergic status. The uptake of alpha-Gal carrying proteins was significantly higher than the uptake of non-alpha-Gal carrying proteins. Confocal microscopy revealed alpha-Gal carrying proteins scattered around the cytoplasm in most iMDDCs while detection of proteins not carrying alpha-Gal was negligible. Fluorescent detection of protein on SDS-PAGE showed that degradation of alpha-Gal carrying proteins was slower than degradation of non-alpha-Gal carrying proteins. Thus, the presence of alpha-Gal on the protein surface affects both uptake and degradation of the protein, and the results add new knowledge of alpha-Gal as a clinically relevant food allergen.", publisher = "Nature Publishing Group, London", journal = "Scientific Reports", title = "alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells", volume = "8", doi = "10.1038/s41598-018-30887-8" }
Krstić-Ristivojević, M., Grundström, J., Tran, T. A. T., Apostolović, D., Radoi, V., Starkhammar, M., Vukojević, V., Ćirković-Veličković, T., Hamsten, C.,& van Hage, M.. (2018). alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells. in Scientific Reports Nature Publishing Group, London., 8. https://doi.org/10.1038/s41598-018-30887-8
Krstić-Ristivojević M, Grundström J, Tran TAT, Apostolović D, Radoi V, Starkhammar M, Vukojević V, Ćirković-Veličković T, Hamsten C, van Hage M. alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells. in Scientific Reports. 2018;8. doi:10.1038/s41598-018-30887-8 .
Krstić-Ristivojević, Maja, Grundström, Jeanette, Tran, Thi Anh Thu, Apostolović, Danijela, Radoi, Vlad, Starkhammar, Maria, Vukojević, Vladana, Ćirković-Veličković, Tanja, Hamsten, Carl, van Hage, Marianne, "alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells" in Scientific Reports, 8 (2018), https://doi.org/10.1038/s41598-018-30887-8 . .