Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine
2018
Аутори
Đorđević, Neda O.Todorović, Nevena M.
Novaković, Irena T.
Pezo, Lato
Pejin, Boris
Maras, Vesna
Tešević, Vele
Pajović, Snezana B.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Screens of antioxidant activity (AA) of various natural products have been a focus of the research community worldwide. This work aimed to differentiate selected samples of Merlot wines originated from Montenegro, with regard to phenolic profile and antioxidant capacity studied by survival rate, total sulfhydryl groups and activities of glutathione peroxidase (GPx), glutathione reductase and catalase in H2O2-stressed Saccharomyces cerevisiae cells. In this study, DPPH assay was also performed. Higher total phenolic content leads to an enhanced AA under both conditions. The same trend was observed for catechin and gallic acid, the most abundant phenolics in the examined wine samples. Finally, the findings of an Artificial Neural Network (ANN) model were in a good agreement (r(2) = 0.978) with the experimental data. All tested samples exhibited a protective effect in H2O2-stressed yeast cells. Pre-treatment with examined wines increased survival in H2O2-stressed cells and shifted antioxi...dative defense towards GPx-mediated defense. Finally, sensitivity analysis of obtained ANN model highlights the complexity of the impact that variations in the concentrations of specific phenolic components have on the antioxidant defense system.
Кључне речи:
red wine / polyphenolics / antioxidant activity / Saccharomyces cerevisiae / artificial neural networkИзвор:
Molecules, 2018, 23, 8Издавач:
- Mdpi, Basel
Финансирање / пројекти:
- Ћелијске и молекулске основе малигних и кардиоваскуларних обољења-клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
- Биоактивни природни производи самониклих, гајених и јестивих биљака: одређивање структура и активности (RS-MESTD-Basic Research (BR or ON)-172053)
DOI: 10.3390/molecules23081971
ISSN: 1420-3049
PubMed: 30087228
WoS: 000445295500136
Scopus: 2-s2.0-85052383450
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Đorđević, Neda O. AU - Todorović, Nevena M. AU - Novaković, Irena T. AU - Pezo, Lato AU - Pejin, Boris AU - Maras, Vesna AU - Tešević, Vele AU - Pajović, Snezana B. PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2222 AB - Screens of antioxidant activity (AA) of various natural products have been a focus of the research community worldwide. This work aimed to differentiate selected samples of Merlot wines originated from Montenegro, with regard to phenolic profile and antioxidant capacity studied by survival rate, total sulfhydryl groups and activities of glutathione peroxidase (GPx), glutathione reductase and catalase in H2O2-stressed Saccharomyces cerevisiae cells. In this study, DPPH assay was also performed. Higher total phenolic content leads to an enhanced AA under both conditions. The same trend was observed for catechin and gallic acid, the most abundant phenolics in the examined wine samples. Finally, the findings of an Artificial Neural Network (ANN) model were in a good agreement (r(2) = 0.978) with the experimental data. All tested samples exhibited a protective effect in H2O2-stressed yeast cells. Pre-treatment with examined wines increased survival in H2O2-stressed cells and shifted antioxidative defense towards GPx-mediated defense. Finally, sensitivity analysis of obtained ANN model highlights the complexity of the impact that variations in the concentrations of specific phenolic components have on the antioxidant defense system. PB - Mdpi, Basel T2 - Molecules T1 - Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine VL - 23 IS - 8 DO - 10.3390/molecules23081971 ER -
@article{ author = "Đorđević, Neda O. and Todorović, Nevena M. and Novaković, Irena T. and Pezo, Lato and Pejin, Boris and Maras, Vesna and Tešević, Vele and Pajović, Snezana B.", year = "2018", abstract = "Screens of antioxidant activity (AA) of various natural products have been a focus of the research community worldwide. This work aimed to differentiate selected samples of Merlot wines originated from Montenegro, with regard to phenolic profile and antioxidant capacity studied by survival rate, total sulfhydryl groups and activities of glutathione peroxidase (GPx), glutathione reductase and catalase in H2O2-stressed Saccharomyces cerevisiae cells. In this study, DPPH assay was also performed. Higher total phenolic content leads to an enhanced AA under both conditions. The same trend was observed for catechin and gallic acid, the most abundant phenolics in the examined wine samples. Finally, the findings of an Artificial Neural Network (ANN) model were in a good agreement (r(2) = 0.978) with the experimental data. All tested samples exhibited a protective effect in H2O2-stressed yeast cells. Pre-treatment with examined wines increased survival in H2O2-stressed cells and shifted antioxidative defense towards GPx-mediated defense. Finally, sensitivity analysis of obtained ANN model highlights the complexity of the impact that variations in the concentrations of specific phenolic components have on the antioxidant defense system.", publisher = "Mdpi, Basel", journal = "Molecules", title = "Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine", volume = "23", number = "8", doi = "10.3390/molecules23081971" }
Đorđević, N. O., Todorović, N. M., Novaković, I. T., Pezo, L., Pejin, B., Maras, V., Tešević, V.,& Pajović, S. B.. (2018). Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine. in Molecules Mdpi, Basel., 23(8). https://doi.org/10.3390/molecules23081971
Đorđević NO, Todorović NM, Novaković IT, Pezo L, Pejin B, Maras V, Tešević V, Pajović SB. Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine. in Molecules. 2018;23(8). doi:10.3390/molecules23081971 .
Đorđević, Neda O., Todorović, Nevena M., Novaković, Irena T., Pezo, Lato, Pejin, Boris, Maras, Vesna, Tešević, Vele, Pajović, Snezana B., "Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine" in Molecules, 23, no. 8 (2018), https://doi.org/10.3390/molecules23081971 . .