Tween 80-solubilized Newcastle disease virus prepared as a water-in-oil-in-water vaccine
Abstract
The Newcastle disease virus (NDV) was solubilized with 10% w/v Tween 80 and inactivated with 0.05% v/v formalin. The average molecular mass of the released antigenic subunits was 307 kD. The rested vaccine was prepared in the form of a water-in-oil-in-water emulsion (WOWE) vaccine. The oil-to-aqueous ratio was 1:2. The solubilized NDV was administered alone or built in a tetravalent WOWE vaccine. A dose of the monovalent vaccine containing an equivalent of 44.7 mu l of detergent-treated NDV-allantoic fluid (NDV-AF) was sufficient for the complete protection of the commercially available chickens vaccinated at the age of 5 wk and challenged 7 wk later. The anti-NDV-free chickens, vaccinated at 4 wk of age and challenged 2 wk postvaccination, were 100% and 73% protected by a vaccinal dose containing 178.6 and 89.3 yl of detergent-treated NDV-AF, respectively. Commercially available light pullers, primary vaccinated with live lentogenic NDV vaccine, generated a protective level of NDV ant...ibodies after revaccination with WOWE vaccine containing 89.3 mu l of detergent treated NDV-AF. Laying hens were revaccinated under field conditions at the beginning of the laying cycle by the terravalent vaccine. A vaccinal dose/bird containing 11.2 mu l of detergent-treated NDV-AF elicited a long-lasting high level of NDV neutralizing antibodies.
Keywords:
chicken / inactivated vaccine / laying hens / Newcastle disease virus / polyvalent vaccine / puller / split-off vaccine / water-in-oil-in-water vaccineSource:
Avian Diseases, 1996, 40, 1, 193-201Publisher:
- Amer Assoc Avian Pathologists, Athens
DOI: 10.2307/1592389
ISSN: 0005-2086
PubMed: 8713034
WoS: A1996UC03800025
Scopus: 2-s2.0-0029691216
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Cajavec, S AU - Bidin, Z AU - Sladić, Dušan AU - Pokric, B PY - 1996 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2240 AB - The Newcastle disease virus (NDV) was solubilized with 10% w/v Tween 80 and inactivated with 0.05% v/v formalin. The average molecular mass of the released antigenic subunits was 307 kD. The rested vaccine was prepared in the form of a water-in-oil-in-water emulsion (WOWE) vaccine. The oil-to-aqueous ratio was 1:2. The solubilized NDV was administered alone or built in a tetravalent WOWE vaccine. A dose of the monovalent vaccine containing an equivalent of 44.7 mu l of detergent-treated NDV-allantoic fluid (NDV-AF) was sufficient for the complete protection of the commercially available chickens vaccinated at the age of 5 wk and challenged 7 wk later. The anti-NDV-free chickens, vaccinated at 4 wk of age and challenged 2 wk postvaccination, were 100% and 73% protected by a vaccinal dose containing 178.6 and 89.3 yl of detergent-treated NDV-AF, respectively. Commercially available light pullers, primary vaccinated with live lentogenic NDV vaccine, generated a protective level of NDV antibodies after revaccination with WOWE vaccine containing 89.3 mu l of detergent treated NDV-AF. Laying hens were revaccinated under field conditions at the beginning of the laying cycle by the terravalent vaccine. A vaccinal dose/bird containing 11.2 mu l of detergent-treated NDV-AF elicited a long-lasting high level of NDV neutralizing antibodies. PB - Amer Assoc Avian Pathologists, Athens T2 - Avian Diseases T1 - Tween 80-solubilized Newcastle disease virus prepared as a water-in-oil-in-water vaccine VL - 40 IS - 1 SP - 193 EP - 201 DO - 10.2307/1592389 ER -
@article{ author = "Cajavec, S and Bidin, Z and Sladić, Dušan and Pokric, B", year = "1996", abstract = "The Newcastle disease virus (NDV) was solubilized with 10% w/v Tween 80 and inactivated with 0.05% v/v formalin. The average molecular mass of the released antigenic subunits was 307 kD. The rested vaccine was prepared in the form of a water-in-oil-in-water emulsion (WOWE) vaccine. The oil-to-aqueous ratio was 1:2. The solubilized NDV was administered alone or built in a tetravalent WOWE vaccine. A dose of the monovalent vaccine containing an equivalent of 44.7 mu l of detergent-treated NDV-allantoic fluid (NDV-AF) was sufficient for the complete protection of the commercially available chickens vaccinated at the age of 5 wk and challenged 7 wk later. The anti-NDV-free chickens, vaccinated at 4 wk of age and challenged 2 wk postvaccination, were 100% and 73% protected by a vaccinal dose containing 178.6 and 89.3 yl of detergent-treated NDV-AF, respectively. Commercially available light pullers, primary vaccinated with live lentogenic NDV vaccine, generated a protective level of NDV antibodies after revaccination with WOWE vaccine containing 89.3 mu l of detergent treated NDV-AF. Laying hens were revaccinated under field conditions at the beginning of the laying cycle by the terravalent vaccine. A vaccinal dose/bird containing 11.2 mu l of detergent-treated NDV-AF elicited a long-lasting high level of NDV neutralizing antibodies.", publisher = "Amer Assoc Avian Pathologists, Athens", journal = "Avian Diseases", title = "Tween 80-solubilized Newcastle disease virus prepared as a water-in-oil-in-water vaccine", volume = "40", number = "1", pages = "193-201", doi = "10.2307/1592389" }
Cajavec, S., Bidin, Z., Sladić, D.,& Pokric, B.. (1996). Tween 80-solubilized Newcastle disease virus prepared as a water-in-oil-in-water vaccine. in Avian Diseases Amer Assoc Avian Pathologists, Athens., 40(1), 193-201. https://doi.org/10.2307/1592389
Cajavec S, Bidin Z, Sladić D, Pokric B. Tween 80-solubilized Newcastle disease virus prepared as a water-in-oil-in-water vaccine. in Avian Diseases. 1996;40(1):193-201. doi:10.2307/1592389 .
Cajavec, S, Bidin, Z, Sladić, Dušan, Pokric, B, "Tween 80-solubilized Newcastle disease virus prepared as a water-in-oil-in-water vaccine" in Avian Diseases, 40, no. 1 (1996):193-201, https://doi.org/10.2307/1592389 . .
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