Electroanalytical Approach for Vitamin B-12 Quantification Based on Its Oxidation at Boron Doped Diamond Electrode
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In this paper electrochemical behavior and sensitive determination of vitamin B12 was studied by using boron doped diamond (BDD) electrode. Cyclic voltammetry and square wave voltammetry were used to investigate the performance of BDD electrode for this purpose. The calibration curve, constructed by plotting oxidation peak current against concentration, obtained in Britton-Robinson buffer solution at pH 2.0 was linear over the wide concentration range of 2-35 mu M with detection limit of 0.7 mu M. Influence of most common interferences compounds was investigated and proposed procedure was successfully applied for quantification of vitamin B12 in real samples, with satisfactory recovery (98-105%). (C) 2016 The Electrochemical Society. All rights reserved.
Source:Journal of the Electrochemical Society, 2016, 163, 7
- Electrochemical Soc Inc, Pennington