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Vezivanje glikozilfosfatidilinozitola(GPI-a) za membranske proteine eritrocita pod dejstvom insulina

dc.creatorTomašević, Nenad
dc.creatorNikolić, Milan
dc.creatorNiketić, Vesna P.
dc.date.accessioned2018-11-22T00:04:31Z
dc.date.available2018-11-22T00:04:31Z
dc.date.issued2002
dc.identifier.issn0352-5139
dc.identifier.urihttps://cherry.chem.bg.ac.rs/handle/123456789/230
dc.description.abstractIn this work GPI binding to membrane proteins from erythrocytes of insulinoma patients for whom prolonged hyperinsulinism and hypoglycemia were characteristic, as well as from normal erythrocytes incubated with supraphysiological concentrations of insulin were analyzed. In the RBCs from insulinoma patients, covalent GPI binding to red cell membrane proteins in the spectrin/ankyrin region, band 4.1 and two proteins of molecular mass of 115 and 110 kD was demonstrated. In erythrocytes incubated with insulin label was associated with band 4.1 and two proteins of molecular mass of 115 and 110 kD. Extraction studies showed that the 100-kD proteins are unrelated to band 3 since they were found in Triton- prepared cytoskeleton. To our knowledge this is the first demonstration of such a modification of red cell skeletal proteins, and the first demonstration of post-translation GPI binding to red cell skeletal proteins in response to insulin. A mechanism proposed for GPI binding to red cell skeletal proteins as well as the relevance of these results for physiological disorders that are characterized by hyperinsulinism are briefly discussed.en
dc.description.abstractU ovom radu ispitivano je vezivanje GPI-a za membranske proteine eritrocita pacijenata obolelih od insulinoma, za koje su karakteristični dugotrajni hiperinsulinizam i hipoglikemija, kao i u normalnim eritrocitima inkubiranim sa suprafiziološkim koncentracijama insulina. Nađeno je da u eritrocitima pacijenata dolazi do kovalentnog vezivanja GPI-a za membranske proteine eritrocita i to u oblasti spektrina i ankirina, za traku 4.1. i dva proteina molekulskih masa 115 i 110kD. U eritrocitima inkubiranim sa insulinom GPI se vezuje za traku 4.1. i dva proteina molekulskih masa 115 i 110 kD. Utvrđeno je da proteini mase 100 kD ne potiču od trake 3, jer su detektovani u citoskeletnoj frakciji zaostaloj posle ekstrakcije membranskih proteina rastvorom Triton-a. U ovom radu je prvi puta detektovana modifikacija citoskeletnih proteina eritrocita vezivanjem GPI-a, kao i post-translaciono vezivanje GPI-a za citoskeletne proteine eritrocita pod uticajem insulina. Ukratko je diskutovan mehanizam vezivanja GPI-a za proteine citoskeleta eritrocita, kao i značaj dobijenih rezultata za razumevanje fizioloških poremećaja u hiperinsulinizmu.sr
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceJournal of the Serbian Chemical Society
dc.subjectinsulinen
dc.subjectGPIen
dc.subjecterythrocytesen
dc.subjectmembranesen
dc.subjecthyperinsulinismen
dc.subjecthypoglycemiaen
dc.titleInsulin-induced glycosylphosphatidylinositol (GPI) binding to red cell membrane proteinsen
dc.titleVezivanje glikozilfosfatidilinozitola(GPI-a) za membranske proteine eritrocita pod dejstvom insulinasr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractТомашевић, Ненад; Николић, Милан; Никетић, Весна П.; Везивање гликозилфосфатидилинозитола(ГПИ-а) за мембранске протеине еритроцита под дејством инсулина; Везивање гликозилфосфатидилинозитола(ГПИ-а) за мембранске протеине еритроцита под дејством инсулина;
dc.citation.volume67
dc.citation.issue12
dc.citation.spage819
dc.citation.epage824
dc.identifier.wos000180192500004
dc.identifier.doi10.2298/JSC0212819T
dc.citation.other67(12): 819-824
dc.citation.rankM23
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-0036981088
dc.identifier.fulltexthttps://cherry.chem.bg.ac.rs/bitstream/id/9277/228.pdf
dc.identifier.rcubKon_244


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