Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina
Само за регистроване кориснике
2016
Аутори
Minić, Simeon L.Stanić-Vučinić, Dragana
Mihailović-Vesić, Jelena
Krstić-Ristivojević, Maja
Nikolić, Milan
Ćirković-Veličković, Tanja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
C-phycocyanin, the major protein of cyanobacteria Spirulina, possesses significant antioxidant, anti-cancer, anti-inflammatory and immunomodulatory effects, ascribed to covalently attached linear tetrapyrrole chromophore phycocyanobilin. There are no literature data about structure and biological activities of released peptides with bound chromophore in C-phycocyanin digest. This study aims to identify chromopeptides obtained after pepsin digestion of C-phycocyanin and to examine their bioactivities. C-phycocyanin is rapidly digested by pepsin in simulated gastric fluid. The structure of released chromopeptides was analyzed by high resolution tandem mass spectrometry and peptides varying in size from 2 to 13 amino acid residues were identified in both subunits of C-phycocyanin. Following separation by HPLC, chromopeptides were analyzed for potential bioactivities. It was shown that all five chromopeptide fractions have significant antioxidant and metal-chelating activities and show cyt...otoxic effect on human cervical adenocarcinoma and epithelial colonic cancer cell lines. In addition, chromopeptides protect human erythrocytes from free radical-induced hemolysis in antioxidative capacity dependant manner. There was a positive correlation between antioxidative potency and other biological activities of chromopeptides. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin whose activity is mostly related to the antioxidative potency provided by chromophore. (C) 2016 Elsevier B.V. All rights reserved.
Кључне речи:
C-phycocyanin / Phycocyanobilin / Digestibility / Peptides / BioactivityИзвор:
Journal of Proteomics, 2016, 147, 132-139Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Молекуларне особине и модификације неких респираторних и нутритивних алергена (RS-MESTD-Basic Research (BR or ON)-172024)
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-FP7-256716)
Напомена:
- Peer-reviewed manuscript: http://cherry.chem.bg.ac.rs/handle/123456789/3444
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3445
DOI: 10.1016/j.jprot.2016.03.043
ISSN: 1874-3919
PubMed: 27084687
WoS: 000383942500015
Scopus: 2-s2.0-84963972252
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Minić, Simeon L. AU - Stanić-Vučinić, Dragana AU - Mihailović-Vesić, Jelena AU - Krstić-Ristivojević, Maja AU - Nikolić, Milan AU - Ćirković-Veličković, Tanja PY - 2016 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2313 AB - C-phycocyanin, the major protein of cyanobacteria Spirulina, possesses significant antioxidant, anti-cancer, anti-inflammatory and immunomodulatory effects, ascribed to covalently attached linear tetrapyrrole chromophore phycocyanobilin. There are no literature data about structure and biological activities of released peptides with bound chromophore in C-phycocyanin digest. This study aims to identify chromopeptides obtained after pepsin digestion of C-phycocyanin and to examine their bioactivities. C-phycocyanin is rapidly digested by pepsin in simulated gastric fluid. The structure of released chromopeptides was analyzed by high resolution tandem mass spectrometry and peptides varying in size from 2 to 13 amino acid residues were identified in both subunits of C-phycocyanin. Following separation by HPLC, chromopeptides were analyzed for potential bioactivities. It was shown that all five chromopeptide fractions have significant antioxidant and metal-chelating activities and show cytotoxic effect on human cervical adenocarcinoma and epithelial colonic cancer cell lines. In addition, chromopeptides protect human erythrocytes from free radical-induced hemolysis in antioxidative capacity dependant manner. There was a positive correlation between antioxidative potency and other biological activities of chromopeptides. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin whose activity is mostly related to the antioxidative potency provided by chromophore. (C) 2016 Elsevier B.V. All rights reserved. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Proteomics T1 - Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina VL - 147 SP - 132 EP - 139 DO - 10.1016/j.jprot.2016.03.043 ER -
@article{ author = "Minić, Simeon L. and Stanić-Vučinić, Dragana and Mihailović-Vesić, Jelena and Krstić-Ristivojević, Maja and Nikolić, Milan and Ćirković-Veličković, Tanja", year = "2016", abstract = "C-phycocyanin, the major protein of cyanobacteria Spirulina, possesses significant antioxidant, anti-cancer, anti-inflammatory and immunomodulatory effects, ascribed to covalently attached linear tetrapyrrole chromophore phycocyanobilin. There are no literature data about structure and biological activities of released peptides with bound chromophore in C-phycocyanin digest. This study aims to identify chromopeptides obtained after pepsin digestion of C-phycocyanin and to examine their bioactivities. C-phycocyanin is rapidly digested by pepsin in simulated gastric fluid. The structure of released chromopeptides was analyzed by high resolution tandem mass spectrometry and peptides varying in size from 2 to 13 amino acid residues were identified in both subunits of C-phycocyanin. Following separation by HPLC, chromopeptides were analyzed for potential bioactivities. It was shown that all five chromopeptide fractions have significant antioxidant and metal-chelating activities and show cytotoxic effect on human cervical adenocarcinoma and epithelial colonic cancer cell lines. In addition, chromopeptides protect human erythrocytes from free radical-induced hemolysis in antioxidative capacity dependant manner. There was a positive correlation between antioxidative potency and other biological activities of chromopeptides. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin whose activity is mostly related to the antioxidative potency provided by chromophore. (C) 2016 Elsevier B.V. All rights reserved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Proteomics", title = "Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina", volume = "147", pages = "132-139", doi = "10.1016/j.jprot.2016.03.043" }
Minić, S. L., Stanić-Vučinić, D., Mihailović-Vesić, J., Krstić-Ristivojević, M., Nikolić, M.,& Ćirković-Veličković, T.. (2016). Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina. in Journal of Proteomics Elsevier Science Bv, Amsterdam., 147, 132-139. https://doi.org/10.1016/j.jprot.2016.03.043
Minić SL, Stanić-Vučinić D, Mihailović-Vesić J, Krstić-Ristivojević M, Nikolić M, Ćirković-Veličković T. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina. in Journal of Proteomics. 2016;147:132-139. doi:10.1016/j.jprot.2016.03.043 .
Minić, Simeon L., Stanić-Vučinić, Dragana, Mihailović-Vesić, Jelena, Krstić-Ristivojević, Maja, Nikolić, Milan, Ćirković-Veličković, Tanja, "Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina" in Journal of Proteomics, 147 (2016):132-139, https://doi.org/10.1016/j.jprot.2016.03.043 . .