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Različite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně]

dc.creatorBogdanović, Jelena
dc.creatorProdanović, Radivoje
dc.creatorMilosavić, Nenad
dc.creatorProdanović, Olivera
dc.creatorRadotić, Ksenija
dc.date.accessioned2018-11-22T00:09:17Z
dc.date.available2018-11-22T00:09:17Z
dc.date.issued2006
dc.identifier.issn0354-4664
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/237
dc.description.abstractActivity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Feisoform was not present in a detectable amount.en
dc.description.abstractProučavana je aktivnost i izoenzimski sastav superoksid dismutaze u apoplastu i ekstraktu celih četina omorike [Picea omorika (Panč.) Purkyně]. Ukupna aktivnost superoksid dismutaze u solubilnoj frakciji ekstrakta četina je bila značajno veća od aktivnosti superoksid dismutaze u apoplastu. U ekstraktu celih četina nađene su nekoliko kiselih i dve sporo-migrirajuće bazne izoforme. Vanćelijska superoksid dismutaza sadrži samo jednu ekstremno kiselu izoformu. Koristeći specifične inhibitore, Cu/Zn- i Mn- superoksid dismutazne forme su identifikovane u ekstraktu celih četina, dok je u apoplastu nađena samo Mn- superoksid dismutaza. Feizoforma nije prisutna u merljivoj koncentraciji.sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/143043/RS//
dc.rightsopenAccess
dc.sourceArchives of biological sciences
dc.subjectantioxidative responseen
dc.subjectenzyme activityen
dc.subjectfree radicalsen
dc.subjectisoelectrofocusingen
dc.titleMultiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]en
dc.titleRazličite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně]sr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractПродановић, Радивоје; Милосавић, Н.; Богдановић, Јелена; Радотић, Ксенија; Продановић, Оливера;
dc.citation.volume58
dc.citation.issue4
dc.citation.spage211
dc.citation.epage214
dc.identifier.doi10.2298/ABS0604211B
dc.citation.other58(4): 211-214
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://cherry.chem.bg.ac.rs/bitstream/id/9341/235.pdf
dc.identifier.rcubKon_432


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