The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?
Само за регистроване кориснике
2017
Аутори
Hadzibrahimovic, MirzetaSužnjević, Desanka
Pastor, Ferenc
Antic, Tijana Cvetic
Žižić, Milan
Zakrzewska, Joanna
Živić, Miroslav
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 A mu mol/g(FW), and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation. Polarographic measurements of mycelium with low concentrations (0.1-1 mM) of V(V), that only allows the presence of monomer, showed that fungal mycelia removes around 27% of V(V) from the extracellular solution. Uptake was saturated at 104 +/- 2 A mu mol/g(FW) which indicates excellent bioaccumulation capability of P. blakesleeanus. EPR, V-51 NMR and polarographic experiments showed no indications of any measurable extracellular complexation of V(V)... monomer with fungal exudates, reduction by the mycelium or adsorption to the cell wall. Therefore, in contrast to vanadium oligomers, vanadate monomer interactions with the mycelium are restricted to its transport into the fungal cell, probably by a phosphate transporter.
Кључне речи:
Bioaccumulation / Fungi / Polarography / Reduction / Uptake / VanadiumИзвор:
Antonie van Leeuwenhoek = International Journal of General and Molecular Microbiology, 2017, 110, 3, 365-373Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-MESTD-Basic Research (BR or ON)-173040)
DOI: 10.1007/s10482-016-0808-0
ISSN: 0003-6072
PubMed: 27896685
WoS: 000394451200009
Scopus: 2-s2.0-84997724751
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Hadzibrahimovic, Mirzeta AU - Sužnjević, Desanka AU - Pastor, Ferenc AU - Antic, Tijana Cvetic AU - Žižić, Milan AU - Zakrzewska, Joanna AU - Živić, Miroslav PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2415 AB - The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 A mu mol/g(FW), and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation. Polarographic measurements of mycelium with low concentrations (0.1-1 mM) of V(V), that only allows the presence of monomer, showed that fungal mycelia removes around 27% of V(V) from the extracellular solution. Uptake was saturated at 104 +/- 2 A mu mol/g(FW) which indicates excellent bioaccumulation capability of P. blakesleeanus. EPR, V-51 NMR and polarographic experiments showed no indications of any measurable extracellular complexation of V(V) monomer with fungal exudates, reduction by the mycelium or adsorption to the cell wall. Therefore, in contrast to vanadium oligomers, vanadate monomer interactions with the mycelium are restricted to its transport into the fungal cell, probably by a phosphate transporter. PB - Springer, Dordrecht T2 - Antonie van Leeuwenhoek = International Journal of General and Molecular Microbiology T1 - The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake? VL - 110 IS - 3 SP - 365 EP - 373 DO - 10.1007/s10482-016-0808-0 ER -
@article{ author = "Hadzibrahimovic, Mirzeta and Sužnjević, Desanka and Pastor, Ferenc and Antic, Tijana Cvetic and Žižić, Milan and Zakrzewska, Joanna and Živić, Miroslav", year = "2017", abstract = "The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 A mu mol/g(FW), and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation. Polarographic measurements of mycelium with low concentrations (0.1-1 mM) of V(V), that only allows the presence of monomer, showed that fungal mycelia removes around 27% of V(V) from the extracellular solution. Uptake was saturated at 104 +/- 2 A mu mol/g(FW) which indicates excellent bioaccumulation capability of P. blakesleeanus. EPR, V-51 NMR and polarographic experiments showed no indications of any measurable extracellular complexation of V(V) monomer with fungal exudates, reduction by the mycelium or adsorption to the cell wall. Therefore, in contrast to vanadium oligomers, vanadate monomer interactions with the mycelium are restricted to its transport into the fungal cell, probably by a phosphate transporter.", publisher = "Springer, Dordrecht", journal = "Antonie van Leeuwenhoek = International Journal of General and Molecular Microbiology", title = "The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?", volume = "110", number = "3", pages = "365-373", doi = "10.1007/s10482-016-0808-0" }
Hadzibrahimovic, M., Sužnjević, D., Pastor, F., Antic, T. C., Žižić, M., Zakrzewska, J.,& Živić, M.. (2017). The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?. in Antonie van Leeuwenhoek = International Journal of General and Molecular Microbiology Springer, Dordrecht., 110(3), 365-373. https://doi.org/10.1007/s10482-016-0808-0
Hadzibrahimovic M, Sužnjević D, Pastor F, Antic TC, Žižić M, Zakrzewska J, Živić M. The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?. in Antonie van Leeuwenhoek = International Journal of General and Molecular Microbiology. 2017;110(3):365-373. doi:10.1007/s10482-016-0808-0 .
Hadzibrahimovic, Mirzeta, Sužnjević, Desanka, Pastor, Ferenc, Antic, Tijana Cvetic, Žižić, Milan, Zakrzewska, Joanna, Živić, Miroslav, "The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?" in Antonie van Leeuwenhoek = International Journal of General and Molecular Microbiology, 110, no. 3 (2017):365-373, https://doi.org/10.1007/s10482-016-0808-0 . .