Mercury-free and modification-free electroanalytical approach towards bromazepam and alprazolam sensing: A facile and efficient assay for their quantification in pharmaceuticals using boron-doped diamond electrodes
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2017
Authors
Samiec, PetrŠovrc, Ľubomír
Stanković, Dalibor
Vojs, Marian
Marton, Marian
Navrátilová, Zuzana
Article (Published version)
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Show full item recordAbstract
The boron-doped diamond electrodes (BDDEs) with different dopant levels were applied as advanced, mercury-free and modification-free electroanalytical platforms for simple and reliable quantification of benzodiazepines, bromazepam (BZ) and alprazolam (ALZ). Cyclic voltammetric measurements revealed that the electrode reactions of both analytes were manifested by one irreversible and diffusion-controlled reduction peak at -1.10V in Britton-Robinson buffer of pH 11 for BZ using lab-made BDDE with 1000 ppm B/C ratio and -0.84V in BR buffer of pH 5 for ALZ using commercial BDDE with 1000 ppm B/C ratio. Differential pulse voltammetry was used for construction of calibration curves for BZ and ALZ, respectively, with the analytical parameters as follows: the linear dynamic ranges of 1 x 10(-6)-1 x 10(-4) and 8 x 10(-7)-1 x 10(-4) mol/L, the detection limit of 3.1 x 10(-7) and 6.4 x 10(-7) mol/L and the excellent repeatability with the relative standard deviation below 3% for both drugs. The d...eveloped methods were applied to analysis of the pharmaceutical tablets with the recoveries from 97.33 to 100.85% for BZ and from 94.14 to 101.32% for ALZ. The usage of BDDEs as advanced electrochemical sensors in drug analysis represents a facile and effective analytical approach which may replace mercury-based sensors and chemically modified electrodes in previous benzodiazepines sensing.
Keywords:
Benzodiazepines / Boron-doped diamond electrode / Electrochemical sensor / Differential pulse voltammetry / Pharmaceutical tabletSource:
Sensors and Actuators B: Chemical, 2017, 245, 963-971Publisher:
- Elsevier Science Sa, Lausanne
Funding / projects:
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-FP7-621375)
- STU Grant scheme
- Student grant competition (University of Ostrava) [SGS07/PrF/2015, SGS03/PrF/2016]
- Grant Agency of the Slovak Republic [1/0489/16]
- Slovak Research and Development Agency [APVV-0365-12]
Note:
- Peer-reviewed manuscript: http://cherry.chem.bg.ac.rs/handle/123456789/3080
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3081
DOI: 10.1016/j.snb.2017.02.023
ISSN: 0925-4005
WoS: 000398760700113
Scopus: 2-s2.0-85013001578
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Inovacioni centar / Innovation CentreTY - JOUR AU - Samiec, Petr AU - Šovrc, Ľubomír AU - Stanković, Dalibor AU - Vojs, Marian AU - Marton, Marian AU - Navrátilová, Zuzana PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2442 AB - The boron-doped diamond electrodes (BDDEs) with different dopant levels were applied as advanced, mercury-free and modification-free electroanalytical platforms for simple and reliable quantification of benzodiazepines, bromazepam (BZ) and alprazolam (ALZ). Cyclic voltammetric measurements revealed that the electrode reactions of both analytes were manifested by one irreversible and diffusion-controlled reduction peak at -1.10V in Britton-Robinson buffer of pH 11 for BZ using lab-made BDDE with 1000 ppm B/C ratio and -0.84V in BR buffer of pH 5 for ALZ using commercial BDDE with 1000 ppm B/C ratio. Differential pulse voltammetry was used for construction of calibration curves for BZ and ALZ, respectively, with the analytical parameters as follows: the linear dynamic ranges of 1 x 10(-6)-1 x 10(-4) and 8 x 10(-7)-1 x 10(-4) mol/L, the detection limit of 3.1 x 10(-7) and 6.4 x 10(-7) mol/L and the excellent repeatability with the relative standard deviation below 3% for both drugs. The developed methods were applied to analysis of the pharmaceutical tablets with the recoveries from 97.33 to 100.85% for BZ and from 94.14 to 101.32% for ALZ. The usage of BDDEs as advanced electrochemical sensors in drug analysis represents a facile and effective analytical approach which may replace mercury-based sensors and chemically modified electrodes in previous benzodiazepines sensing. PB - Elsevier Science Sa, Lausanne T2 - Sensors and Actuators B: Chemical T1 - Mercury-free and modification-free electroanalytical approach towards bromazepam and alprazolam sensing: A facile and efficient assay for their quantification in pharmaceuticals using boron-doped diamond electrodes VL - 245 SP - 963 EP - 971 DO - 10.1016/j.snb.2017.02.023 ER -
@article{ author = "Samiec, Petr and Šovrc, Ľubomír and Stanković, Dalibor and Vojs, Marian and Marton, Marian and Navrátilová, Zuzana", year = "2017", abstract = "The boron-doped diamond electrodes (BDDEs) with different dopant levels were applied as advanced, mercury-free and modification-free electroanalytical platforms for simple and reliable quantification of benzodiazepines, bromazepam (BZ) and alprazolam (ALZ). Cyclic voltammetric measurements revealed that the electrode reactions of both analytes were manifested by one irreversible and diffusion-controlled reduction peak at -1.10V in Britton-Robinson buffer of pH 11 for BZ using lab-made BDDE with 1000 ppm B/C ratio and -0.84V in BR buffer of pH 5 for ALZ using commercial BDDE with 1000 ppm B/C ratio. Differential pulse voltammetry was used for construction of calibration curves for BZ and ALZ, respectively, with the analytical parameters as follows: the linear dynamic ranges of 1 x 10(-6)-1 x 10(-4) and 8 x 10(-7)-1 x 10(-4) mol/L, the detection limit of 3.1 x 10(-7) and 6.4 x 10(-7) mol/L and the excellent repeatability with the relative standard deviation below 3% for both drugs. The developed methods were applied to analysis of the pharmaceutical tablets with the recoveries from 97.33 to 100.85% for BZ and from 94.14 to 101.32% for ALZ. The usage of BDDEs as advanced electrochemical sensors in drug analysis represents a facile and effective analytical approach which may replace mercury-based sensors and chemically modified electrodes in previous benzodiazepines sensing.", publisher = "Elsevier Science Sa, Lausanne", journal = "Sensors and Actuators B: Chemical", title = "Mercury-free and modification-free electroanalytical approach towards bromazepam and alprazolam sensing: A facile and efficient assay for their quantification in pharmaceuticals using boron-doped diamond electrodes", volume = "245", pages = "963-971", doi = "10.1016/j.snb.2017.02.023" }
Samiec, P., Šovrc, Ľ., Stanković, D., Vojs, M., Marton, M.,& Navrátilová, Z.. (2017). Mercury-free and modification-free electroanalytical approach towards bromazepam and alprazolam sensing: A facile and efficient assay for their quantification in pharmaceuticals using boron-doped diamond electrodes. in Sensors and Actuators B: Chemical Elsevier Science Sa, Lausanne., 245, 963-971. https://doi.org/10.1016/j.snb.2017.02.023
Samiec P, Šovrc Ľ, Stanković D, Vojs M, Marton M, Navrátilová Z. Mercury-free and modification-free electroanalytical approach towards bromazepam and alprazolam sensing: A facile and efficient assay for their quantification in pharmaceuticals using boron-doped diamond electrodes. in Sensors and Actuators B: Chemical. 2017;245:963-971. doi:10.1016/j.snb.2017.02.023 .
Samiec, Petr, Šovrc, Ľubomír, Stanković, Dalibor, Vojs, Marian, Marton, Marian, Navrátilová, Zuzana, "Mercury-free and modification-free electroanalytical approach towards bromazepam and alprazolam sensing: A facile and efficient assay for their quantification in pharmaceuticals using boron-doped diamond electrodes" in Sensors and Actuators B: Chemical, 245 (2017):963-971, https://doi.org/10.1016/j.snb.2017.02.023 . .