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Peptidomics of an in vitro digested alpha-Gal carrying protein revealed IgE-reactive peptides
dc.creator | Apostolović, Danijela | |
dc.creator | Krstić-Ristivojević, Maja | |
dc.creator | Mihailović-Vesić, Jelena | |
dc.creator | Starkhammar, Maria | |
dc.creator | Ćirković-Veličković, Tanja | |
dc.creator | Hamsten, Carl | |
dc.creator | van Hage, Marianne | |
dc.date.accessioned | 2018-11-22T00:40:34Z | |
dc.date.available | 2018-11-22T00:40:34Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/2486 | |
dc.description.abstract | The mammalian carbohydrate galactose-alpha 1,3-galactose (alpha-Gal) causes a novel form of food allergy, red meat allergy, where patients experience severe allergic reactions several hours after red meat consumption. Here we explored gastric digestion of alpha-Gal glycoproteins using an in vitro model. Bovine thyroglobulin (BTG), a typical alpha-Gal carrying glycoprotein, was digested with pepsin. The resulting peptides were characterised by SDS PAGE, immunoblot and ImmunoCAP using sera from 20 red meat allergic patients. During pepsinolysis of BTG, a wide range of peptide bands was observed of which 14 to 17 kDa peptides remained stable throughout the gastric phase. The presence of the alpha-Gal epitope on the obtained peptides was demonstrated by an anti-alpha-Gal antibody and IgE from red meat allergic patients. The alpha-Gal digests were able to inhibit up to 86% of IgE reactivity to BTG. Importantly, basophil activation test demonstrated that the allergenic activity of BTG was retained after digestion in all four tested patients. Mass spectrometry-based peptidomics revealed that these peptides represent mostly internal and C-terminal parts of the protein, where the most potent IgE-binding alpha-Gal residues were identified at Asn(1756), Asn(1850) and Asn(2231). Thus allergenic a-Gal epitopes are stable to pepsinolysis, reinforcing their role as clinically relevant food allergens. | en |
dc.publisher | Nature Publishing Group, London | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172024/RS// | |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/256716/EU// | |
dc.relation | EAACI | |
dc.relation | Magnus Bergvall Foundation | |
dc.relation | Hesselman Foundation | |
dc.relation | Swedish Research Council | |
dc.relation | Konsul ThC Bergh Foundation | |
dc.relation | Swedish Cancer and Allergy Foundation | |
dc.relation | King Gustaf V 80th Birthday Foundation | |
dc.relation | Swedish Association for Allergology | |
dc.relation | Stockholm County Council (ALF project) | |
dc.relation | Swedish Asthma and Allergy Associations Research Foundation | |
dc.relation | Swedish Heart-Lung Foundation | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Scientific Reports | |
dc.title | Peptidomics of an in vitro digested alpha-Gal carrying protein revealed IgE-reactive peptides | en |
dc.type | article | |
dc.rights.license | BY | |
dcterms.abstract | Старкхаммар, М.; Крстић-Ристивојевић, Маја; Ћирковић-Величковић, Тања; Михаиловић Весић, Јелена; Aпостоловиц, Д.; Хамстен, Ц.; ван Хаге, М.; | |
dc.citation.volume | 7 | |
dc.identifier.wos | 000405425000005 | |
dc.identifier.doi | 10.1038/s41598-017-05355-4 | |
dc.citation.other | 7: | |
dc.citation.rank | M21 | |
dc.identifier.pmid | 28701697 | |
dc.description.other | Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3020] | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-85024102637 | |
dc.identifier.fulltext | https://cherry.chem.bg.ac.rs/bitstream/id/9440/2484.pdf |