Characterization and effects of binding of food-derived bioactive phycocyanobilin to bovine serum albumin
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AutoriMinić, Simeon L.
Radomirović, Mirjana Ž.
Milčić, Miloš K.
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Phycocyanobilin (PCB) is a blue tetrapyrrole chromophore of C-phycocyanin, the main protein of the microalga Spirulina, with numerous proven health-related benefits. We examined binding of PCB to bovine serum albumin (BSA) and how it affects protein and ligand stability. Protein fluorescence quenching and microscale thermophoresis demonstrated high-affinity binding (K-a = 2 x 10(6) M-1). Spectroscopic titration with molecular docking analysis revealed two binding sites on BSA, at the inter-domain cleft and at subdomain IB, while CD spectroscopy indicated stereo-selective binding of the P conformer of the pigment to the protein. The PCB protein complex showed increased thermal stability. Although complex formation partly masked the antioxidant properties of PCB and BSA, a mutually protective effect against free radical-induced oxidation was found. BSA could be suitable for delivery of PCB as a food colorant or bioactive component. Our results also highlight subtle differences between PC...B binding to bovine vs. human serum albumin. (C) 2017 Elsevier Ltd. All rights reserved.
Ključne reči:Spirulina / Phycocyanobilin / Bovine serum albumin / Binding / Stability / Antioxidant
Izvor:Food Chemistry, 2018, 239, 1090-1099
- Molekularne osobine i modifikacije nekih respiratornih i nutritivnih alergena (RS-172024)
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-256716)