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dc.creatorRadan, Mila
dc.creatorCarev, Ivana
dc.creatorTešević, Vele
dc.creatorPoliteo, Olivera
dc.creatorČulić, Vedrana Čikeš
dc.date.accessioned2018-11-22T00:41:09Z
dc.date.available2018-11-22T00:41:09Z
dc.date.issued2017
dc.identifier.issn1612-1872
dc.identifier.urihttps://cherry.chem.bg.ac.rs/handle/123456789/2524
dc.description.abstractCentaurea ragusina L., an endemic Croatian plant species, revealed a good cytotoxic activity of aqueous extracts (AE) on human bladder (T24) and human glioblastoma (A1235) cancer cell lines. The chemical constituents were tentatively identified using high performance liquid chromatography HPLC-DAD/ ESI-TOF-MS in negative ionization mode. The main compounds of herba extract were sesquiterpene lactones: solstitialin A 3,13-diacetate and epoxyrepdiolide; organic acid: quinic acid. The main compounds of flower extract were organic acids: quinic acid, citric acid, and malic acid; sesquiterpene lactone: cynaropicrin; phenolic compounds: chlorogenic acid and phenylpropanoid: syringin. The AE of C. ragusina were investigated for correlation of their effects on human bladder (T24) and human glioblastoma (A1235) cancer cell lines using the MTT assay. Although both extracts showed significant dose-and time-dependent cytotoxic activity against both cancer cell lines, the flower extract exhibited slightly higher activity. In order to determine type of cell death induced by treatment, cell lines were exposed subsequently to a treatment with both flower and herba AE. The majority of the cells died by induced apoptosis treatment. Flower AE (26.25%), compared to a leaf AE (22.15%) showed slightly higher percentage of an apoptosis in T24 cells, when compared to a non-treated cells (0.04%).en
dc.publisherWiley-V C H Verlag Gmbh, Weinheim
dc.relationCroatian Science Foundation [IP-2014-09-6897]
dc.rightsrestrictedAccess
dc.sourceChemistry and Biodiversity
dc.subjectCentaurea ragusinaen
dc.subjectCytotoxic activitiesen
dc.subjectApoptotic activitiesen
dc.subjectMTTen
dc.subjectSesquiterpene lactoneen
dc.titleQualitative HPLC-DAD/ESI-TOF-MS Analysis, Cytotoxic, and Apoptotic Effects of Croatian Endemic Centaurea ragusina L. Aqueous Extractsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractТешевић, Веле; Царев, Ивана; Радан, Мила; Цулиц, Ведрана Цикес; Политео, Оливера;
dc.citation.volume14
dc.citation.issue9
dc.identifier.wos000410558700004
dc.identifier.doi10.1002/cbdv.201700099
dc.citation.other14(9): e1700099
dc.citation.rankM22
dc.description.otherPeer-reviewed manuscript: [http://cherry.chem.bg.ac.rs/handle/123456789/3245]
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-85026497390
dc.identifier.rcubKon_3340


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