Anti-encephalitogenic effects of cucumber leaf extract
Само за регистроване кориснике
2017
Аутори
Jevtic, BojanDjedovic, Neda
Stanisavljevic, Suzana
Gašić, Uroš M.
Mišić, Danijela
Despotović, Jovana
Samardžić, Jelena
Miljković, Đorđe
Timotijević, Gordana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further inves...tigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved.
Кључне речи:
Cucumber / Cucurbitacin / T cell / Macrophage / Autoimmunity / Experimental autoimmune encephalomyelitisИзвор:
Journal of Functional Foods, 2017, 37, 249-262Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Корелација структуре и особина природних и синтетичких молекула и њихових комплекса са металима (RS-172017)
- Молекуларни механизми одговора биљака на абиотички стрес-улога транскрипционих фактора и малих РНК и анализа генетичког диверзитета биљних култура од интереса за пољопривреду и биотехнологију (RS-173005)
- Комплексне болести као модел систем за проучавање модулације фенотипа-структурна и функционална анализа молекуларних биомаркера (RS-173008)
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-173013)
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-173024)
- Ћелијски и молекулски механизми опоравка пацова од експерименталног аутоимунског енцефаломијелитиса (RS-173035)
DOI: 10.1016/j.jff.2017.07.060
ISSN: 1756-4646
WoS: 000412863400025
Scopus: 2-s2.0-85026788295
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Jevtic, Bojan AU - Djedovic, Neda AU - Stanisavljevic, Suzana AU - Gašić, Uroš M. AU - Mišić, Danijela AU - Despotović, Jovana AU - Samardžić, Jelena AU - Miljković, Đorđe AU - Timotijević, Gordana PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2537 AB - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Functional Foods T1 - Anti-encephalitogenic effects of cucumber leaf extract VL - 37 SP - 249 EP - 262 DO - 10.1016/j.jff.2017.07.060 ER -
@article{ author = "Jevtic, Bojan and Djedovic, Neda and Stanisavljevic, Suzana and Gašić, Uroš M. and Mišić, Danijela and Despotović, Jovana and Samardžić, Jelena and Miljković, Đorđe and Timotijević, Gordana", year = "2017", abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Functional Foods", title = "Anti-encephalitogenic effects of cucumber leaf extract", volume = "37", pages = "249-262", doi = "10.1016/j.jff.2017.07.060" }
Jevtic, B., Djedovic, N., Stanisavljevic, S., Gašić, U. M., Mišić, D., Despotović, J., Samardžić, J., Miljković, Đ.,& Timotijević, G.. (2017). Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods Elsevier Science Bv, Amsterdam., 37, 249-262. https://doi.org/10.1016/j.jff.2017.07.060
Jevtic B, Djedovic N, Stanisavljevic S, Gašić UM, Mišić D, Despotović J, Samardžić J, Miljković Đ, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods. 2017;37:249-262. doi:10.1016/j.jff.2017.07.060 .
Jevtic, Bojan, Djedovic, Neda, Stanisavljevic, Suzana, Gašić, Uroš M., Mišić, Danijela, Despotović, Jovana, Samardžić, Jelena, Miljković, Đorđe, Timotijević, Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" in Journal of Functional Foods, 37 (2017):249-262, https://doi.org/10.1016/j.jff.2017.07.060 . .