Anti-encephalitogenic effects of cucumber leaf extract
Authorized Users Only
2017
Authors
Jevtic, BojanDjedovic, Neda
Stanisavljevic, Suzana
Gašić, Uroš M.
Mišić, Danijela
Despotović, Jovana
Samardžić, Jelena
Miljković, Đorđe
Timotijević, Gordana
Article (Published version)
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Show full item recordAbstract
Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further inves...tigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved.
Keywords:
Cucumber / Cucurbitacin / T cell / Macrophage / Autoimmunity / Experimental autoimmune encephalomyelitisSource:
Journal of Functional Foods, 2017, 37, 249-262Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-MESTD-Basic Research (BR or ON)-172017)
- The Role of Transcription Factors and Small RNAs in Abiotic Stress Response in Plants and Genetic Diversity of Plant Species Important for Agriculture and Biotechnology (RS-MESTD-Basic Research (BR or ON)-173005)
- Complex diseases as a model system for phenotype modulation- structural and functional analysis of molecular biomarkers (RS-MESTD-Basic Research (BR or ON)-173008)
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Cellular and molecular mechanisms of recovery of rats from experimental autoimmune encephalomyelitis (RS-MESTD-Basic Research (BR or ON)-173035)
DOI: 10.1016/j.jff.2017.07.060
ISSN: 1756-4646
WoS: 000412863400025
Scopus: 2-s2.0-85026788295
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Jevtic, Bojan AU - Djedovic, Neda AU - Stanisavljevic, Suzana AU - Gašić, Uroš M. AU - Mišić, Danijela AU - Despotović, Jovana AU - Samardžić, Jelena AU - Miljković, Đorđe AU - Timotijević, Gordana PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2537 AB - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Functional Foods T1 - Anti-encephalitogenic effects of cucumber leaf extract VL - 37 SP - 249 EP - 262 DO - 10.1016/j.jff.2017.07.060 ER -
@article{ author = "Jevtic, Bojan and Djedovic, Neda and Stanisavljevic, Suzana and Gašić, Uroš M. and Mišić, Danijela and Despotović, Jovana and Samardžić, Jelena and Miljković, Đorđe and Timotijević, Gordana", year = "2017", abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Functional Foods", title = "Anti-encephalitogenic effects of cucumber leaf extract", volume = "37", pages = "249-262", doi = "10.1016/j.jff.2017.07.060" }
Jevtic, B., Djedovic, N., Stanisavljevic, S., Gašić, U. M., Mišić, D., Despotović, J., Samardžić, J., Miljković, Đ.,& Timotijević, G.. (2017). Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods Elsevier Science Bv, Amsterdam., 37, 249-262. https://doi.org/10.1016/j.jff.2017.07.060
Jevtic B, Djedovic N, Stanisavljevic S, Gašić UM, Mišić D, Despotović J, Samardžić J, Miljković Đ, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods. 2017;37:249-262. doi:10.1016/j.jff.2017.07.060 .
Jevtic, Bojan, Djedovic, Neda, Stanisavljevic, Suzana, Gašić, Uroš M., Mišić, Danijela, Despotović, Jovana, Samardžić, Jelena, Miljković, Đorđe, Timotijević, Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" in Journal of Functional Foods, 37 (2017):249-262, https://doi.org/10.1016/j.jff.2017.07.060 . .