Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan
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Gojgić-Cvijović, Gordana D.
Jakovljević, Dragica M.
Stefanović Kojić, Jovana R.
Harju, Inka Elina
Vrvić, Miroslav M.
Article (Published version)
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Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NM...R) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan. (C) 2017 Elsevier B.V. All rights reserved.
Keywords:Polluted environments / Exopolysaccharides / Brachybacterium paraconglomeratum
Source:International Journal of Biological Macromolecules, 2017, 104, 311-321
- Elsevier Science Bv, Amsterdam
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
- FCUB ERA
- Japan International Cooperation Agency (JICA)
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/2965