Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base

2017
Authors
Petric, MarijaSubotic, Angelina
Jevremovic, Sladana
Trifunović-Momcilov, Milana

Tadić, Vojin

Grujić, Marica
Vujčić, Zoran

Article (Published version)
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The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was vis...ible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris.
Keywords:
aryl esterase / esterase / F. meleagris / peroxidase / somatic embryogenesisSource:
Archives of biological sciences, 2017, 69, 4, 619-625Publisher:
- Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
Funding / projects:
- Biotechnology in vitro - crop, medicinal and endangered plant species (RS-173015)
- Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)
DOI: 10.2298/ABS161130007P
ISSN: 0354-4664
WoS: 000413353300006
Scopus: 2-s2.0-85031690343
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Petric, Marija AU - Subotic, Angelina AU - Jevremovic, Sladana AU - Trifunović-Momcilov, Milana AU - Tadić, Vojin AU - Grujić, Marica AU - Vujčić, Zoran PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2543 AB - The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was visible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris. PB - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd T2 - Archives of biological sciences T1 - Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base VL - 69 IS - 4 SP - 619 EP - 625 DO - 10.2298/ABS161130007P ER -
@article{ author = "Petric, Marija and Subotic, Angelina and Jevremovic, Sladana and Trifunović-Momcilov, Milana and Tadić, Vojin and Grujić, Marica and Vujčić, Zoran", year = "2017", abstract = "The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was visible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris.", publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd", journal = "Archives of biological sciences", title = "Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base", volume = "69", number = "4", pages = "619-625", doi = "10.2298/ABS161130007P" }
Petric, M., Subotic, A., Jevremovic, S., Trifunović-Momcilov, M., Tadić, V., Grujić, M.,& Vujčić, Z.. (2017). Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. in Archives of biological sciences Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 69(4), 619-625. https://doi.org/10.2298/ABS161130007P
Petric M, Subotic A, Jevremovic S, Trifunović-Momcilov M, Tadić V, Grujić M, Vujčić Z. Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. in Archives of biological sciences. 2017;69(4):619-625. doi:10.2298/ABS161130007P .
Petric, Marija, Subotic, Angelina, Jevremovic, Sladana, Trifunović-Momcilov, Milana, Tadić, Vojin, Grujić, Marica, Vujčić, Zoran, "Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base" in Archives of biological sciences, 69, no. 4 (2017):619-625, https://doi.org/10.2298/ABS161130007P . .