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Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo
dc.creator | Ljubetič, Ajasja | |
dc.creator | Lapenta, Fabio | |
dc.creator | Gradišar, Helena | |
dc.creator | Drobnak, Igor | |
dc.creator | Aupič, Jana | |
dc.creator | Strmšek, Žiga | |
dc.creator | Lainšček, Duško | |
dc.creator | Hafner-Bratkovič, Iva | |
dc.creator | Majerle, Andreja | |
dc.creator | Krivec, Nuša | |
dc.creator | Benčina, Mojca | |
dc.creator | Pisanski, Tomaž | |
dc.creator | Ćirković-Veličković, Tanja | |
dc.creator | Round, Adam | |
dc.creator | José María Carazo | |
dc.creator | Melero, Roberto | |
dc.creator | Jerala, Roman | |
dc.date.accessioned | 2018-11-22T00:41:31Z | |
dc.date.available | 2018-11-22T00:41:31Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1087-0156 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/2549 | |
dc.description.abstract | Polypeptides and polynucleotides are natural programmable biopolymers that can self-assemble into complex tertiary structures. We describe a system analogous to designed DNA nanostructures in which protein coiled-coil (CC) dimers serve as building blocks for modular de novo design of polyhedral protein cages that efficiently self-assemble in vitro and in vivo. We produced and characterized gt 20 single-chain protein cages in three shapes-tetrahedron, four-sided pyramid, and triangular prism-with the largest containing gt 700 amino-acid residues and measuring 11 nm in diameter. Their stability and folding kinetics were similar to those of natural proteins. Solution small-angle X-ray scattering (SAXS), electron microscopy (EM), and biophysical analysis confirmed agreement of the expressed structures with the designs. We also demonstrated self-assembly of a tetrahedral structure in bacteria, mammalian cells, and mice without evidence of inflammation. A semi-automated computational design platform and a toolbox of CC building modules are provided to enable the design of protein cages in any polyhedral shape. | en |
dc.publisher | Nature Publishing Group, New York | |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/256716/EU// | |
dc.relation | Slovenian Research Agency [P4-0176, N4-0037, J4-5528, L4-6812, J3-7034, BI-US/17-18-051] | |
dc.relation | NVIDIA Corporation for the donation of the Quadro [GP100 GPU] | |
dc.relation | ERANET SynBio project Bioorigami [ERASYNBIO1-006] | |
dc.relation | ICGEB [CRP/SLO14-03] | |
dc.relation | iNEXT - Horizon 2020 Programme of the EU [PID1771, PID2706, PID1824, VID3987] | |
dc.relation | COST actions [CM1304, CM1306] | |
dc.rights | restrictedAccess | |
dc.source | Nature Biotechnology | |
dc.title | Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Јерала, Роман; Роунд, Aдам; Бенцина, Мојца; Лаинсцек, Дуско; Хафнер-Братковиц, Ива; Мајерле, Aндреја; Лапента, Фабио; Љубетиц, Aјасја; Градисар, Хелена; Дробнак, Игор; Aупиц, Јана; Стрмсек, Зига; Кривец, Нуса; Ћирковић-Величковић, Тања; Мелеро, Роберто; Писански, Томаз; Мариа Царазо, Јосе; | |
dc.citation.volume | 35 | |
dc.citation.issue | 11 | |
dc.citation.spage | 1094 | |
dc.identifier.wos | 000414856200026 | |
dc.identifier.doi | 10.1038/nbt.3994 | |
dc.citation.other | 35(11): 1094-1101 | |
dc.citation.rank | M21 | |
dc.identifier.pmid | 29035374 | |
dc.description.other | Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3212] | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-85033600678 |