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dc.creatorMilivojević, Ana
dc.creatorĆorović, Marija
dc.creatorCarević, Milica
dc.creatorBanjanac, Katarina
dc.creatorVujisić, Ljubodrag V.
dc.creatorVeličković, Dusan
dc.creatorBezbradica, Dejan
dc.date.accessioned2018-11-22T00:41:39Z
dc.date.available2018-11-22T00:41:39Z
dc.date.issued2017
dc.identifier.issn1369-703X
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/2558
dc.description.abstractSolubility and stability of flavonoid glycosides, valuable natural constituents of cosmetics and pharmaceuticals, could be improved by lipase-catalyzed acylation. Focus of this study was on development of eco-friendly process for the production of flavonoid acetates. By using phloridzin as model compound and triacetin as acetyl donor and solvent, 100% conversion and high productivity (23.32 gl(-1) day(-1)) were accomplished. Complete conversions of two other glycosylated flavonoids, naringin and esculin, in solvent-free system were achieved, as well. Comprehensive kinetic mechanism based on two con-secutive mono-substrate reactions was established where first one represents formation of flavonoid monoacetate and within second reaction diacetate is being produced from monoacetate. Both steps were regarded as reversible Michaelis-Menten reactions without inhibition. Apparent kinetic parameters for two consecutive reactions (V-m constants for substrates and products and Km constants for forward and reverse reactions) were estimated for three examined acetyl acceptors and excellent fitting of experimental data (R-2 gt 0.97) was achieved. Obtained results showed that derived kinetic model could be applicable for solvent-free esterifications of different flavonoid glycosides. It was valid for entire transesterification course (72 h of reaction) which, combined with complete conversions and green character of synthesis, represents firm basis for further process development. (C) 2017 Elsevier B.V. All rights reserved.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172053/RS//
dc.rightsrestrictedAccess
dc.sourceBiochemical Engineering Journal
dc.subjectFlavonoid acetylationen
dc.subjectLipaseen
dc.subjectSolvent-freeen
dc.subjectKinetic parametersen
dc.subjectCOPASIen
dc.titleHighly efficient enzymatic acetylation of flavonoids: Development of solvent-free process and kinetic evaluationen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЦоровиц, Марија; Безбрадица, Дејан; Вујисић, Љубодраг; Бањанац, Катарина; Велицковиц, Дусан; Царевиц, Милица; Миливојевиц, Aна;
dc.citation.volume128
dc.citation.spage106
dc.citation.epage115
dc.identifier.wos000417008900012
dc.identifier.doi10.1016/j.bej.2017.09.018
dc.citation.other128: 106-115
dc.citation.rankM21
dc.description.otherSupplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2974]
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-85030172007
dc.identifier.rcubKon_3374


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