Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture
Mao, Angelo S.
Mooney, David J.
Weitz, David A.
Article (Accepted Version)
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Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.
Keywords:alginate / cells / encapsulation / microfluidics / microgels
Source:Advanced Healthcare Materials, 2015, 4, 11, 1628-1633
- Wiley-Blackwell, Hoboken
- National Institute of Health [R01 EB014703, P01GM096971]
- National Science Foundation [DMR-1310266]
- Deutsche Forschungsgemeinschaft (DFG)
- Fulbright Foundation [N0009552407]
- Harvard MRSEC [DMR-0820484]
- This is the peer-reviewed version of the article: Utech, S., Prodanovic, R., Mao, A.S., Ostafe, R., Mooney, D.J., Weitz, D.A., 2015. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 4, 1628–1633. https://doi.org/10.1002/adhm.201500021
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3383