Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture

Utech, Stefanie; Prodanović, Radivoje; Mao, Angelo S.; Ostafe, Raluca; Mooney, David J.; Weitz, David A.

doi:10.1002/adhm.201500021

2015

nihms699213.pdf (1.134Mb)

Authors

Utech, Stefanie
Prodanović, Radivoje

Mao, Angelo S.
Ostafe, Raluca

Mooney, David J.
Weitz, David A.

Article (Accepted Version)

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Abstract

Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.

Keywords:

alginate / cells / encapsulation / microfluidics / microgels

Source:
Advanced Healthcare Materials, 2015, 4, 11, 1628-1633

Publisher:

Wiley-Blackwell, Hoboken

Funding / projects:

National Institute of Health [R01 EB014703, P01GM096971]
National Science Foundation [DMR-1310266]
Deutsche Forschungsgemeinschaft (DFG)
Fulbright Foundation [N0009552407]
Harvard MRSEC [DMR-0820484]

Note:

This is the peer-reviewed version of the article: Utech, S., Prodanovic, R., Mao, A.S., Ostafe, R., Mooney, D.J., Weitz, D.A., 2015. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 4, 1628–1633. https://doi.org/10.1002/adhm.201500021
Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3383

DOI: 10.1002/adhm.201500021

ISSN: 2192-2640

PubMed: 26039892

WoS: 000359382600003

Scopus: 2-s2.0-84938747686

[ Google Scholar ]


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	167

TY  - JOUR
AU  - Utech, Stefanie
AU  - Prodanović, Radivoje
AU  - Mao, Angelo S.
AU  - Ostafe, Raluca
AU  - Mooney, David J.
AU  - Weitz, David A.
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2785
AB  - Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.
PB  - Wiley-Blackwell, Hoboken
T2  - Advanced Healthcare Materials
T1  - Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture
VL  - 4
IS  - 11
SP  - 1628
EP  - 1633
DO  - 10.1002/adhm.201500021
ER  -

@article{
author = "Utech, Stefanie and Prodanović, Radivoje and Mao, Angelo S. and Ostafe, Raluca and Mooney, David J. and Weitz, David A.",
year = "2015",
abstract = "Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Advanced Healthcare Materials",
title = "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture",
volume = "4",
number = "11",
pages = "1628-1633",
doi = "10.1002/adhm.201500021"
}

Utech, S., Prodanović, R., Mao, A. S., Ostafe, R., Mooney, D. J.,& Weitz, D. A.. (2015). Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. in Advanced Healthcare Materials
Wiley-Blackwell, Hoboken., 4(11), 1628-1633.
https://doi.org/10.1002/adhm.201500021

Utech S, Prodanović R, Mao AS, Ostafe R, Mooney DJ, Weitz DA. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. in Advanced Healthcare Materials. 2015;4(11):1628-1633.
doi:10.1002/adhm.201500021 .

Utech, Stefanie, Prodanović, Radivoje, Mao, Angelo S., Ostafe, Raluca, Mooney, David J., Weitz, David A., "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture" in Advanced Healthcare Materials, 4, no. 11 (2015):1628-1633,
https://doi.org/10.1002/adhm.201500021 . .