Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture

Utech, Stefanie; Prodanović, Radivoje; Mao, Angelo S.; Ostafe, Raluca; Mooney, David J.; Weitz, David A.

doi:10.1002/adhm.201500021

2015

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Authors

Utech, Stefanie
Prodanović, Radivoje

Mao, Angelo S.
Ostafe, Raluca

Mooney, David J.
Weitz, David A.

Article (Accepted Version)

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Abstract

Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.

Keywords:

alginate / cells / encapsulation / microfluidics / microgels

Source:
Advanced Healthcare Materials, 2015, 4, 11, 1628-1633

Publisher:

Wiley-Blackwell, Hoboken

Projects:

National Institute of Health [R01 EB014703, P01GM096971]
National Science Foundation [DMR-1310266]
Deutsche Forschungsgemeinschaft (DFG)
Fulbright Foundation [N0009552407]
Harvard MRSEC [DMR-0820484]

Note:

This is the peer-reviewed version of the article: Utech, S., Prodanovic, R., Mao, A.S., Ostafe, R., Mooney, D.J., Weitz, D.A., 2015. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 4, 1628–1633. https://doi.org/10.1002/adhm.201500021
Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3383