Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture
Authors
Utech, StefanieProdanović, Radivoje

Mao, Angelo S.
Ostafe, Raluca

Mooney, David J.
Weitz, David A.
Article (Accepted Version)
Metadata
Show full item recordAbstract
Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.
Keywords:
alginate / cells / encapsulation / microfluidics / microgelsSource:
Advanced Healthcare Materials, 2015, 4, 11, 1628-1633Publisher:
- Wiley-Blackwell, Hoboken
Funding / projects:
- National Institute of Health [R01 EB014703, P01GM096971]
- National Science Foundation [DMR-1310266]
- Deutsche Forschungsgemeinschaft (DFG)
- Fulbright Foundation [N0009552407]
- Harvard MRSEC [DMR-0820484]
Note:
- This is the peer-reviewed version of the article: Utech, S., Prodanovic, R., Mao, A.S., Ostafe, R., Mooney, D.J., Weitz, D.A., 2015. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 4, 1628–1633. https://doi.org/10.1002/adhm.201500021
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3383
DOI: 10.1002/adhm.201500021
ISSN: 2192-2640
PubMed: 26039892
WoS: 000359382600003
Scopus: 2-s2.0-84938747686
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Utech, Stefanie AU - Prodanović, Radivoje AU - Mao, Angelo S. AU - Ostafe, Raluca AU - Mooney, David J. AU - Weitz, David A. PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2785 AB - Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments. PB - Wiley-Blackwell, Hoboken T2 - Advanced Healthcare Materials T1 - Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture VL - 4 IS - 11 SP - 1628 EP - 1633 DO - 10.1002/adhm.201500021 ER -
@article{ author = "Utech, Stefanie and Prodanović, Radivoje and Mao, Angelo S. and Ostafe, Raluca and Mooney, David J. and Weitz, David A.", year = "2015", abstract = "Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.", publisher = "Wiley-Blackwell, Hoboken", journal = "Advanced Healthcare Materials", title = "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture", volume = "4", number = "11", pages = "1628-1633", doi = "10.1002/adhm.201500021" }
Utech, S., Prodanović, R., Mao, A. S., Ostafe, R., Mooney, D. J.,& Weitz, D. A.. (2015). Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. in Advanced Healthcare Materials Wiley-Blackwell, Hoboken., 4(11), 1628-1633. https://doi.org/10.1002/adhm.201500021
Utech S, Prodanović R, Mao AS, Ostafe R, Mooney DJ, Weitz DA. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. in Advanced Healthcare Materials. 2015;4(11):1628-1633. doi:10.1002/adhm.201500021 .
Utech, Stefanie, Prodanović, Radivoje, Mao, Angelo S., Ostafe, Raluca, Mooney, David J., Weitz, David A., "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture" in Advanced Healthcare Materials, 4, no. 11 (2015):1628-1633, https://doi.org/10.1002/adhm.201500021 . .