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Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6
(Wiley-Blackwell Publishing, Inc, Malden, 2009)
Two melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies ...
Hydrophilic-Interaction Planar Chromatography of Some Water-Soluble Co(III) Complexes on Different Adsorbents
(Research Inst Medicinal Plants, Budakalasz, 2009)
The chromatographic behavior of twelve neutral, mixed cobalt(III) complexes of the uns-cis-edda-type has been investigated in six planar chromatographic systems. Four different stationary phases unmodified silica gel, ...
Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species
(Springer, New York, 2006)
Polyketides are a group of bioactive compounds from bacteria, plants, and fungi. To increase the availability of analogs for testing, the active sites of polyketide synthases are often substituted with homologous domains ...
Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation
(Wiley-Blackwell Publishing, Inc, Malden, 2009)
A library of 20 000 transposon (Tn5) mutants of the gram-negative bacterium Pseudomonas putida CA-3 was generated and screened for adverse affects in polyhydroxyalkanoates (PHA) accumulation. Two mutants of interest were ...
Resolution of methyl nonactate by Rhodococcus erythropolis under aerobic and anaerobic conditions
(Amer Chemical Soc, Washington, 2006)
An efficient resolution of methyl nonactate is reported by biotransformation in shake flask cultures of Rhodococcus erythropolis. The equilibrium of the reaction redox system can be manipulated by switching from aerobic ...
A second generation snp-derived Escherichia coli-Streptomyces shuttle expression vector that is generally transferable by conjugation
(Academic Press Inc Elsevier Science, San Diego, 2006)
An Escherichia coli-Streptomyces shuttle vector (pJN100) was constructed, by inserting an origin of transfer (oriT), derived from the E coli broad host range plasmid RK2, into pANT1202, a high-copy-number vector for gene ...
The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate
(Springer, New York, 2008)
Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the ...
Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments
(Wiley-Blackwell, Malden, 2008)
Immobilized bacteria are being assessed by industry for drug delivery, novel fermentation systems and the protection of organisms in harsh environments. Alginate bioreactors containing Streptomyces nodosus were examined ...
High yield preparation of genomic DNA from Streptomyces
(Eaton Publishing Co, Natick, 2003)
High frequency transformation of the amphotericin-producing bacterium Streptomyces nodosus
(Elsevier Science Bv, Amsterdam, 2003)
This study has investigated DNA transformation in the Amphotericin-producing organism Streptomyces nodosus. Amphotericin B is an antifungal drug with severe side effects in humans and the availability of structural variants ...