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dc.creatorGligorijević, Nikola
dc.creatorMinić, Simeon L.
dc.creatorRobajac, Dragana B.
dc.creatorNikolić, Milan
dc.creatorĆirković-Veličković, Tanja
dc.creatorNedić, Olgica
dc.date.accessioned2019-02-13T09:00:29Z
dc.date.available2020-01-22
dc.date.issued2019
dc.identifier.issn0141-8130
dc.identifier.urihttps://cherry.chem.bg.ac.rs/handle/123456789/2825
dc.description.abstractFibrinogen, a protein involved in blood coagulation, is very susceptible to oxidation. Oxidation alters its function and usually makes it more thrombogenic. Bilirubin, an end-product of the haem degradation in vertebrates, is known for its antioxidant properties. The present paper describes interaction between fibrinogen and bilirubin, and the influence of bilirubin on the formation of fibrin and protection against oxidation. The binding constant of 4.5 × 104 M−1 was determined for the fibrinogen/bilirubin complex at 37 °C. There is no change in secondary and tertiary structure of fibrinogen or its thermal stability upon bilirubin binding. The binding site of fibrinogen is not stereospecific for bilirubin and is able to accommodate both bilirubin conformers. A change in absorption maximum of bilirubin occurs upon its interaction with fibrinogen, suggesting an alteration in the conformation of bilirubin to the more cyclic one. Bilirubin exerts antioxidant effect on fibrinogen, preventing its carbonylation and aggregation. The presence of bilirubin induces the formation of fibrin with thicker fibres, as assessed by the coagulation assay. Fibrinogen and bilirubin interact at physiological concentrations, bilirubin may act as an antioxidant for fibrinogen and may modulate an important event in haemostasis, which altogether suggests possible physiological relevance of this interaction.
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173042/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172024/RS//
dc.rightsembargoedAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceInternational Journal of Biological Macromolecules
dc.subjectBinding
dc.subjectCircular dichroism
dc.subjectCoagulation
dc.subjectFluorimetry
dc.subjectProtein oxidation
dc.titleCharacterisation and the effects of bilirubin binding to human fibrinogen
dc.typearticleen
dc.rights.licenseBY-NC-ND
dcterms.abstractНедић, Олгица; Робајац, Драгана Б.; Глигоријевић, Никола; Минић, Симеон Л.; Николић, Милан; Ћирковић-Величковић, Тања;
dc.citation.volume128
dc.citation.spage74
dc.citation.epage79
dc.identifier.wos000463305100010
dc.identifier.doi10.1016/j.ijbiomac.2019.01.124
dc.citation.rankaM21
dc.description.otherThis is the peer-reviewed version of the following article: Gligorijević, Nikola; Minić, Simeon L.; Robajac, Dragana B.; Nikolić, Milan; Ćirković-Veličković, Tanja; Nedić, Olgica, 2019. Characterisation and the effects of bilirubin binding to human fibrinogen. International Journal of Biological Macromolecules. 128, 74-79. [https://doi.org/10.1016/j.ijbiomac.2019.01.124]
dc.description.otherSupplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2937]
dc.type.versionacceptedVersion
dc.identifier.scopus2-s2.0-85060455696
dc.identifier.fulltexthttps://cherry.chem.bg.ac.rs/bitstream/id/6509/10.1016@j.ijbiomac.2019.01.124.pdf


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