Production of biotechnological useful metabolites by mucor racemosus in czapek-dox liquid media supplemented with synthetic detergent
Produkcija biotehnološki korisnih metabolita gljivom mucor racemosus u tečnoj czapek-dox podlozi sa dodatkom sintetičkog deterdženta
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The capacity of native isolate Mucor racemosus to produce several potentially useful metabolites in a liquid Czapek-Dox medium supplemented with powder anionic-type detergent MERIX (Henkel, Serbia) at concentrations of 0.3% (D3) and 0.5% (D5) was examined in this study. The changes of pH values, the total protein content, activities of acid and alkaline invertase, α-amylase, as well as biomass dry weight were evaluated during fungal growth from inoculation until the 16 th day. In addition, the qualitative and quantitative amino acids content of 16 days old fermentation broth was determined by HPLC. D3 considerably enhanced the biomass dry weight (43%), α-amylase activity (49.59%) and the quantity of arginine (40.38%), and also influenced the production of a high amount of proteins (5.32 g/L). D5 significantly enhanced the biomass dry weight (53%), the quantity of arginine (119.09%) and alanine (192.79%) and induced the production of valine, serine and glutamate. In the D5 medium, α-amy...lase retained 100% of its activity. The acid and alkaline invertase activity was moderately inhibited by D3 and D5. The obtained results may have considerable biotechnological, industrial and environmental potential.
Keywords:Amino acids / Amylase / Detergent / Growth / Invertase / Mucor racemosus
Source:Chemical Industry and Chemical Engineering Quarterly, 2018, 24, 3, 209-219
- Beograd : Association of the Chemical Engineers of Serbia