Voltammetric protocol for reliable determination of a platelet aggregation inhibitor dipyridamole on a bare miniaturized boron-doped diamond electrochemical sensor
Authorized Users Only
Article (Published version)
MetadataShow full item record
In present paper, a miniaturized boron-doped diamond electrode (BDDE) was for the first time used as a reliable electrochemical sensor within the novel voltammetric protocol for the rapid and simple determination of dipyridamole (DIP). Cyclic voltammetric studies uncovered that the electrochemical oxidation of DIP was shown to be irreversible and diffusion-controlled process with single and well-developed signal at a potential of +0.45 V in Britton-Robinson buffer at pH 3.0. Using the suitable experimental conditions, the linearity for DIP determination was observed in the concentration ranges of 0.1.5.0 μM and 0.1.6.0 μM with the corresponding limits of detection of 40 nM and 60 nM as well as the intra-day repeatability with relative standard deviation below 5% for differential pulse and square-wave voltammetry, respectively. This protocol was successfully applied to the determination of DIP in pharmaceutical tablets with result in good compliance with that declared by the producer. B...iological pertinence of the developed procedure was presented by analysis of model human urine samples with adequate recoveries. The proposed protocol with a miniaturized BDDE could represent an effective and alternative analytical implement for DIP determination instead of commonly used toxic mercury and chemically-modified electrodes in previously published methods.
Source:Journal of the Electrochemical Society, 2019, 166, 4, B219-B226
- Electrochemical Society Inc.
- National Scholarship Programme of the Slovak Republic for the Support of Mobility of Students, PhD Students, University Teachers, Researches and Artist and the grant Agency of the Slovak Republic (grant No. 1/0489/16 and 1/0463/18)
- Slovak Research and Development Agency (APVV-15-0585)
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-172030)
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-621375)